PCNA, Human Recombinant

• Catalog number
  P1562-100
• Price
  Please ask
• Size
  100 µg

• Synonyms
  Proliferating cell nuclear antigen, MGC8367, Proliferating cell nuclear antigen, Proliferating cell nuclear antigen, PCNA
• Alternative_names
  Proliferating cell nuclear antigen, MGC8367, Proliferating cell nuclear antigen, Proliferating cell nuclear antigen, PCNA
• Description
  A processivity factor for DNA polymerase δ
• Recombinant
  1
• Source
  E. coli
• Purity by SDS PAGE
  >95%
• Assay
  SDS - PAGE
• Molecular Weight
  28 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  Liquid
• Background Information
  PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, Sphase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighbouring tumours. Recombinant human PCNA, was expressed in E. coli and purified by conventional chromatography techniques.
• Amino acid sequence
  aa 1-261
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PCNA  
• Gene symbol
  PCNA, PCNA-AS1
• Short name
  PCNA, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  PCNA, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:8729
• Gene
  PCNA
• Long gene name
  proliferating cell nuclear antigen
• GenBank acession
  • J04718
• Locus
  20p12.3
• Discovery year
  1990-01-15
• Entrez gene record
  5111
• Pubmed identfication
  • 2565339
• VEGA ID
  OTTHUMG00000031798

Gene info

• Identity
  HGNC:37184
• Gene
  PCNA-AS1
• Long gene name
  PCNA antisense RNA 1
• Synonyms gene
  • PCNAAS
  • PCNA-AS
• Synonyms gene name
  • PCNA antisense RNA (non-protein coding)
  • PCNA antisense RNA 1 (non-protein coding)
• Locus
  20p12.3
• Discovery year
  2009-08-18
• Entrez gene record
  100302739
• Pubmed identfication
  • 10488129
  • 24704293
• RefSeq identity
  • NR_028370
• Classification
  • Antisense RNAs

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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