Midkine, human recombinant

• Catalog number
  4479-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  NEGF-2, Neurite Growth-Promoting Factor 2, MK, Neurite outgrowth-promoting protein, Midgestation and kidney protein, Amphiregulin-associated protein, ARAP, Neurite outgrowth-promoting factor 2, FLJ27379, Midkine, MK1, NEGF2.
• Description
  A basic heparin-binding growth factor
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Purity
  ≥98%
• Biological activity
  Determined by its ability to chemoattract human neutrophils using a concentration of 0.1-10 ng/ml.
• Results
  0.1-10 ng/ml
• Molecular Weight
  13.4 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Lyophilized from 0.4X PBS, pH7.4
• Reconstitution Instructions
  Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
• Background Information
  Human Midkine (MK) is a new member of the heparin-binding neurotrophic factor family. MK and PTN (Pleiotrophin) are structural homologs, and are highly conserved among species. MK plays important roles in development and carcinogenesis and has several important biological effects, including promotion of neurite extension and neuronal survival. Recombinant midkine is a 13.4 kDa protein, comprising of 123 amino acid residues.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Midkine  
• Gene symbol
  MDK
• Short name
  Midkine, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Midkine, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:6972
• Gene
  MDK
• Long gene name
  midkine
• Synonyms gene
  • NEGF2
• Synonyms gene name
  • neurite growth-promoting factor 2
• Synonyms
  • MK
  • FLJ27379
• Locus
  11p11.2
• Discovery year
  1991-07-10
• Entrez gene record
  4192
• Pubmed identfication
  • 8406506
• RefSeq identity
  • NM_001012334
• VEGA ID
  OTTHUMG00000150315

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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