HEPES, endotoxin and cell culture tested [CAS# 7365-45-9]

  • Catalog number
    H006-1KG
  • Price
    Please ask
  • Size
    1 kg
  • Availability
    Please contact us to check current availability
  • Long description
    HEPES, endotoxin and cell culture tested [CAS# 7365-45-9]; MW 238.3
  • Product format
    Powder
  • Product category
    Biochemicals
  • Product subcategory
    Buffers and Supplements
  • Shipping method
    Ambient temperature
  • Storage method
    Store at 2˚ to 8˚C.
  • Description
    The CAS or CASRN registry number for chemical substances and identifying chemicals by the Chemical Abstract Service is a worldwide used identification of chemicals. This chemical can be supplied in purities of 98% or 95% purity by caissonlab. Bulk in kg or liter volumes on request. For cells, cell lines and tissues in culture till half confluency.
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Gene target
  • Gene symbol
    IGHV1-45, SNORD115-45, IGLV5-45, RNU6-9, PIRC45, PIRC47, PIRC38
  • Short name
    HEPES
  • Technique
    culture
  • Alternative name
    HEPES, endotoxin and cellular culture tested [CAS# 7365-45-9]
  • Tissue
    cell
Gene info
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 45
  • Locus
    9
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 47
  • Locus
    9
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 38
  • Locus
    9
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
MeSH Data
  • Name
  • Concept
    Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
  • Tree numbers
    • E05.599.495
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