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Category
Primary Antibodies
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Long description
Recognizes a protein of 33-36kDa, identified as the Excision Repair Cross Complementing (ERCC1). The mammalian ERCC1-encoded polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. The structure-specific ERCC1/XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV-induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross-links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.
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Antibody come from
Synthetic peptide derived from human histone H2a.x protein.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Antigen Immunoaffinity Purification
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti ERCC1 (DNA excision repair protein) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Immunohistochemistry (paraffin-embedded)
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Antibody s suited for
Antibody can be used for Western blotting (1-5 µg/ml) and immunohistochemistry (2-5 µg/ml at pH 9.5). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Paull, T.T., et al. ÔA critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damageÕ Curr. Biol., 10, 886-895 (2000)_x000B__x000B_2. Kobayashi, J., et al. ÔNBS1 localizes to gamma-H2AX foci through interaction with the FHA/BRCT domain.Õ Curr. Biol., 12, 1846-1851 (2002)_x000B__x000B_3. Stewart, G.S., et al. ÔMDC1 is a mediator of the mammalian DNA damage checkpoint.Õ Nature, 421, 961-966 (2003)_x000B__x000B_4. Lukas, C., et al. ÔMdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention.Õ EMBO J., 23, 2674-2683 (2004)
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
ERCC1, PRKDC, HLA-DOA
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Short name
Rabbit anti ERCC1 (DNA excision repair protein)
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Technique
Rabbit, anti, dna, antibody to
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Host
Rabbit, Rabbits
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Isotype
not specified
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Label
unlabeled
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Alternative name
production species: rabbit antibody to excision repair cross-complementing rodent repair deficiency, complementation family 1 (includes overlapping antisense sequence) (Desoxyribonucleic acid excision repair protein)
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Alternative technique
rabbit-anti, antibodies
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Alternative to gene target
excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence), COFS4 and RAD10 and UV20, ERCC1 and IDBG-57509 and ENSG00000012061 and 2067, structure-specific DNA binding, nuclei, Ercc1 and IDBG-150201 and ENSMUSG00000003549 and 13870, ERCC1 and IDBG-639466 and ENSBTAG00000017355 and 615637
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