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Also known as
CytokeRatin 8 / KeRatin K8
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Other names
cytokeratin 8; Keratin, type II cytoskeletal 8; keratin, type II cytoskeletal 8; CK-8; keratin-8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8; keratin 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
KRT8; KRT8; K8; KO; CK8; CYK8; K2C8; CARD2; CYK8
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Gene name synonims
KRT8; KRT8; K8; KO; CK8; CYK8; K2C8; CARD2; CYK8
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Other gene names
KRT8; KRT8; K8; KO; CK8; CYK8; K2C8; CARD2; CYK8
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
M20
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Host organism
Mouse (Mus musculus)
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Species reactivity
Human (Homo sapiens), Rabbit (Oryctolagus cuniculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
M20 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells and epithelia of the digestive, respiRatory and urogenital tract. Also most adenocarcinomas derived from these cells are positive. The M20 epitope has been mapped to amino acids 353-367 on the alpha helical rod domain of cytokeRatin 8 (see reference 10).; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius., or in small aliquots the antibody should be stored at -20 degrees Celsius..
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Tested applications
Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps