NKCC1 Antibody SLC12A2

• Catalog number
  R31290
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  NKCC1 SLC12A2
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, IHC-F
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the NKCC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This NKCC1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P55011
• Purity
  Antigen affinity
• Description
  Solute carrier family 12 (sodium/potassium/chloride transporters), member 2, also known as NKCC1 (Na-K-Cl Cotransporter 1), is widely distributed throughout the body, especially in organs that secrete fluids, called exocrine glands. By fluorescence in situ hybridization, this gene is mapped to chromosome 5q23.3. The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. NKCC1 is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene.
• Immunogen
  An amino acid sequence from the N-terminus of human NKCC1 (RIDHYRHTAAQLGEKLLRP) was used as the immunogen for this NKCC1 antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the NKCC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  NKCC1   SLC12A2  
• Gene symbol
  SLC12A2, SLC12A2-DT
• Short name
  Anti-NKCC1 SLC12A2
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to NKCC1 SLC12A2
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:10911
• Gene
  SLC12A2
• Long gene name
  solute carrier family 12 member 2
• Synonyms gene name
  • solute carrier family 12 (sodium/potassium/chloride transporter), member 2
• Synonyms
  • NKCC1
  • BSC
  • BSC2
  • PPP1R141
• Synonyms name
  • bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
  • basolateral Na-K-Cl symporter
  • protein phosphatase 1, regulatory subunit 141
• Locus
  5q23.3
• Discovery year
  1994-02-16
• Entrez gene record
  6558
• Pubmed identfication
  • 7629105
• RefSeq identity
  • NM_001046
• Classification
  • Solute carriers
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000128983

Gene info

• Identity
  HGNC:49565
• Gene
  SLC12A2-DT
• Long gene name
  SLC12A2 divergent transcript
• Synonyms gene
  • LINC01184
• Synonyms gene name
  • long intergenic non-protein coding RNA 1184
• Synonyms
  • FLJ33630
• GenBank acession
  • BX648677
• Locus
  5q23.3
• Discovery year
  2014-01-11
• Entrez gene record
  644873
• Pubmed identfication
  • 24036268
• RefSeq identity
  • NR_015360
• Classification
  • Divergent transcripts
• VEGA ID
  OTTHUMG00000163312

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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