Target of this antibody:
Alternative names of antibody target:
CASP-9 , EC 18.104.22.168 , ICE-like apoptotic protease 6 ,ICE-LAP6 ,Apoptotic protease Mch-6 ,Apoptotic protease-activating factor 3 , Apaf-3
Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase-9/Apaf-1. The complex processes procaspase-9 into a large subunit (35 kDa or 17 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase-9 can also be cleaved in vivo by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.
This antibody reacts with:
Synthetic peptide surrounding amino acid 345 of mouse caspase-9
Concentration of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) protein A purified rabbit anti-caspase-9 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Mouse small intestine and rat kidney tissue lysates can be used as positive controls. The antibody detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved caspase-9.