CytoSelect™ BrdU Cell Proliferation ELISA test kit

  • Catalog number
    CBA-251
  • Price
    Please ask
  • Size
    96 assays
  • We send this reagant on
    Room temperature
  • The temperatureerature for storage is
    Upon receipt, store the 1000X BrdU Solution and Anti-BrdU Monoclonal Antibody at -20ºC. Store all other components at 4ºC.
  • Technical file
    more kit.pdf
  • SDS or safty data sheet
    details (CBA-251) GHS.pdf
  • Type of reagent
    Cell Based Assays
  • Related techniques
    Cell Based Assays, Cell Health Assays, Cell Proliferation Assays, BrdU Cell Proliferation ELISA
  • Use of the reagent
    The CytoSelect™ BrdU Cell Proliferation ELISA test kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody.  When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells.  Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection).  Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU.  The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.
  • Cellular use
    Detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody
  • Protocol information
    BrdU standard included
  • Conjugation and form
    host specific
  • Species compatible to
    human cells and cell lines and other cells
  • Use of this test kit or product
    Molecular biology
  • Antigen
    the sequence of the protein
  • Buffer
    The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,47
  • Molecular weight
    kiloDalton
  • This reagent is purified
    0.98
  • Description
    For cells, cell lines and tissues in culture till half confluency.
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Storage
    Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
  • Group
    Enzyme-Linked Immunosorbent Assay kits
  • Gene target
  • Short name
    CytoSelect™ BrdU Proliferation ELISA test kit
  • Technique
    ELISA test, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests.
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Alternative name
    CytoSelect™ Bromodeoxyuridine cellular expantion Enzyme-linked immunosorbent assay test reagent
  • Alternative technique
    elisas, tests, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Tissue
    cell
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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