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Category
Primary Antibodies
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Long description
Neurons and neuroendocrine cells contain membrane-delimited pools of peptide hormones, biogenic amines, and neurotransmitters with a characteristic electron-dense appearance. These vesicles, which are present throughout the neuroendocrine system and in a variety of neurons, store and release chromogranins and secretogranins, a unique group of acidic, soluble secretory proteins. Chromogranin A, a protein of 439 amino acids which is encoded on chromosome 14, and an apparent molecular weight of 68kDa, is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors, including small cell lung carcinoma.
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Antibody come from
LK2H10 is a Mouse monoclonal IgG1 antibody derived by fusion of NS-1 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with an immunogen that has been isolated from Human pheochromocytoma.
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Other description
Each vial contains 1 ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
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Clone
LK2H10
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Antigen antibody binding interaction
Mouse anti Chromogranin A Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
LK2H10 reacts with the soluble secretory protein Chromogranin A.
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Research interest
Cancer, Neurobiology
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Application
Immunohistochemistry (frozen),Immunohistochemistry (paraffin)
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Antibody s suited for
LK2H10 is useful for immunohistochemistry on paraffin embedded and frozen tissues. Optimal antibody dilution should be determined by titration; recommended dilution 1:10 when applied in the Indirect immunofluorescence method. With the ABC method the dilution can be 5-10x higher. Dilute immediately before use with PBS or TRIS-HCl pH 7,0.
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Storage
Store undiluted antibody at 2-8ºC (until expiry date). For prolonged storage: store aliquots at -20ºC.
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Relevant references
Taupenot, L., Harper, K.L., O’Connor, D.T. (2003). The chromogranin-secretogranin family. N Engl J Med 348, 1134-49. Lloyd, R.V. and Wilson, B.S. (1983). Specific endocrine tissue marker defined by a monoclonal antibody. Science 222, 628-30. Wilson, B.S. and Lloyd, R.V. (1984). Detection of chromagranin in neuroendocrine cells with a monoclonal antibody. Am J Pathol 115, 458-68.
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Protein number
P10645
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus