Target of this antibody:
Alternative names of antibody target:
CTSD , CLN10, CPSD, MGC2311
Cathepsin D is a normal lysosomal protease that is expressed in all cells. It is an aspartyl protease with a pH optimum in the range of 3-5, and contains two N-linked oligosaccharides. Cathepsin D is synthesized as an inactive 52 kDa pro-enzyme. Activation involves the proteolytic removal of the 43 amino acid profragment and an internal cleavage to generate the two-chain form made up of 34 and 14 kDa subunits. Cathepsin D contains the mannose-6-phosphate lysosomal localization signal that targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, Cathepsin D is abnormally processed and is secreted in its 52 kDa precursor form. Numerous clinical studies as well as in vitro evidence sμggest that cathepsin D plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 140 of mouse cathepsins D
Concentration of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-cathepsin D polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot analysis (0.5-4 µg/ml) and in immunoprecipitation. However, the optimal conditions should be determined individually. The purified antibody detects both the 52 kDa proform and the 34 kDa cleaved fragment of Cathepsin D.
Long gene name cathepsin D
Synonyms gene name
- cathepsin D (lysosomal aspartyl protease)
- ceroid-lipofuscinosis, neuronal 10
Discovery year 1986-01-01
Entrez gene record 1509
Havana BLAST/BLAT OTTHUMG00000044760
Locus Specific Databases
- Neuronal Ceroid Lipofuscinoses; NCL Mutations