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Target antigen
SIRT3
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human
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Analyses
WB
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Immunogen
E.coli-derived human SIRT3 recombinant protein (Position: P66-K399). Human SIRT3 shares 86% amino acid (aa) sequence identity with mouse SIRT3.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the SIRT3 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The SIRT3 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
NAD-dependent deacetylase sirtuin-3, mitochondrial also known as SIRT3 is a protein that in humans is encoded by the SIRT3 gene. SIRT3 is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein. It is mapped to 11p15.5. Endogenous SIRT3 is a soluble protein located in the mitochondrial matrix. Overexpression of SIRT3 in cultured cells increases respiration and decreases the production of reactive oxygen species. SIRT3 can function as a mitochondrial deacetylase. In addition to it, human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.
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Related articles
1. "Entrez Gene: SIRT3 sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)" 2. Schwer B, North BJ, Frye RA, Ott M, Verdin E (August 2002). "The human silent information regulator (Sir)2 homologue hSIRT3 is a mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase". J. Cell Biol. 158 (4): 647–57. 3. Onyango P, Celic I, McCaffery JM, Boeke JD, Feinberg AP (October 2002). "SIRT3, a human SIR2 homologue, is an NAD-dependent deacetylase localized to mitochondria". Proc. Natl. Acad. Sci. U.S.A. 99 (21): 13653–8.
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Gene Name
SIRT3
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Protein Name
NAD-dependent protein deacetylase sirtuin-3, mitochondrial
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Gene Full Name
sirtuin 3
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Synonyms
hSIRT 3 antibody|hSIRT3 antibody|Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody|NAD dependent deacetylase sirtuin 3 mitochondrial antibody|NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody|Regulatory protein SIR2 homolog 3 antibody|Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody|Sir 2 like 3 antibody|SIR 2 like protein 3 antibody|SIR 3 antibody|SIR2 L3 antibody|Sir2 like 3 antibody|SIR2 like protein 3 antibody|SIR2-like protein 3 antibody|SIR2L3 antibody|SIR3_HUMAN antibody|SIRT 3 antibody|SIRT3 antibody|Sirtuin 3 antibody|Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody|Sirtuin type 3 antibody|Sirtuin3 antibody
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Uniprot ID
Q9NTG7
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Entrez GeneID
23410
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
