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AAV DJ 8 Helper Free Expression System

AAV DJ 8 Helper Free Expression System is available 8 times from Cellular biology labs labs

VPK-419-DJ-8 | AAV-DJ/8 Helper Free Bicistronic Expression System (Blasticidin) size: 1 test kit | 1,579.78 USD

SDS or safty data sheetmore (VPK-410 through VPK-430-SER6) GHS.pdf
Related techniques Viral Expression, Adeno-Associated Virus (AAV) Expression, AAV Helper Free Complete Expression Systems, AAV-DJ/8 Helper Free Complete Expression Systems
Use of the reagent The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.
Cellular use Complete Expression System contains packaging plasmids, expression vector and GFP control vector
Protocol information pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
Additional lab advice Cloning capacity of expression vector = 2 kb
Buffer The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,51
Short name AAV-DJ/8 Helper Free Bicistronic Expression System (Blasticidin)
Alternative name AAV-DJ/8 Helper Free Bicistronic Expression System (Blasticidin)
1. Gene info
Identity22
GeneAAVS1
Long gene name adeno-associated virus integration site 1
Synonyms
GenBank acession
Locus19q13
Discovery year 1990-09-10
Entrez gene record17
Pubmed identfication
Additional information

VPK-418-DJ-8 | AAV-DJ/8 Helper Free Bicistronic Expression System (GFP) size: 1 test kit | 1,579.78 USD

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VPK-417-DJ-8 | AAV-DJ/8 Helper Free Bicistronic Expression System (Hygro) size: 1 test kit | 1,579.78 USD

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VPK-416-DJ-8 | AAV-DJ/8 Helper Free Bicistronic Expression System (Neo) size: 1 test kit | 1,579.78 USD

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VPK-415-DJ-8 | AAV-DJ/8 Helper Free Bicistronic Expression System (Puro) size: 1 test kit | 1,579.78 USD

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VPK-410-DJ-8 | AAV-DJ/8 Helper Free Expression System size: 1 test kit | 1,579.78 USD

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VPK-400-DJ-8 | AAV-DJ/8 Helper Free Packaging System size: 1 test kit | 1,238.12 USD

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VPK-411-DJ-8 | AAV-DJ/8 Helper Free Promoterless Expression System size: 1 test kit | 1,579.78 USD

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Living modified organisme in this reagent or LMO Not applicable
Antigen a specific protein sequence
This reagent is purified 0.97
Type of reagent Viral Expression
The temperatureerature for storage is -20ºC
Use of this test kit or product Genetics lab
We send this reagant on Blue Ice
Reagent properties AAV-DJ/8 is a mutant of AAV-DJ designed to provide increased uptake in brain tissue, closely mimicking AAV-8 and AAV-9
Species compatible to Humans
Molecular weight in kilo Danlto or kDa
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