Lck Antibody
-
Catalog number3498-30T
-
PricePlease ask
-
Size30 µg
-
-
Antibody TargetLck
-
Target Alternative NameLCK , p56-LCK , p56lck , YT16 , pp58lck , LSK
-
Antibody TypePolyclonal
-
Species ReactivitiesHuman, mouse, rat
-
Immunogen SequenceSynthetic peptide corresponding to the C-terminus of human Lck
-
AccessionP06239
-
Gene Id3932
-
AppearanceColorless liquid
-
FormLiquid
-
Concentration0.5 mg/ml
-
Formulation30 µg (0.5 mg/ml) antigen affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
-
PurificationAffinity purified
-
ApplicationWestern blot, Immunoprecipitation, Immunohistochemistry
-
Application And UsagesWestern blotting (0.5-4 µg/ml), immunoprecipitation (20-25 µg/ml), and Immunohistochemistry (20-25 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 56 kDa Lck protein from samples of human, mouse, rat origins. A low band ~25 kDa can also be detected, presumably to be a cleavage fragment of Lck.
-
DescriptionLck is a membrane associated tyrosine protein kinases. The Lck gene has been shown to undergo rearrangement and overexpression in some murine lymphomas. In human studies, it has been demonstrated that the Lck gene is localized to a site in the genome which undergoes frequent chromosomal abnormalities in lymphomas and neuroblastomas. Resting T cells contain high levels of the Lck protein and mRNA both of which decline upon activation of the T cells. These and other observations sμggest that alterations in Lck expression may contribute to the pathogenesis of some types of neoplastic disease.
-
Storage Condition-20 °C
-
Shipping Conditiongel pack
-
Shelf Life12 months
-
UsageFor Research Use Only! Not to be used in humans.
-
HandlingThe antibody solution should be gently mixed before use.
-
Western Blot VerifiedVerified
-
Immunocytochemistry VerifiedNot Verified
-
Immunofluorescence VerifiedNot Verified
-
Immunoprecipitation VerifiedNot Verified
-
ELISA VerifiedNot Verified
-
ChIP VerifiedNot Verified
-
Flow Cytometry VerifiedNot Verified
-
PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translationanticorps
-
Gene target
-
Gene symbolLCK, LIME1
-
Short nameLck Antibody
-
TechniqueAntibody, antibodies against human proteins, antibodies for
-
IsotypeRabbit IgG
-
Alternative namelymphocyte-specific protein tyrosine kinase (antibody to-)
-
Alternative techniqueantibodies
-
Alternative to gene targetlymphocyte-specific protein tyrosine kinase, IMD22 and LSK and p56lck and pp58lck and YT16, LCK and IDBG-95667 and ENSG00000182866 and 3932, transferase activity, Cell surfaces, Lck and IDBG-191117 and ENSMUSG00000000409 and 16818, LCK and IDBG-644592 and ENSBTAG00000012695 and 508890
-
Gene info
-
Identity
-
Gene
-
Long gene nameLCK proto-oncogene, Src family tyrosine kinase
-
Synonyms gene name
- lymphocyte-specific protein tyrosine kinase
-
GenBank acession
-
Locus
-
Discovery year1986-01-01
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Src family tyrosine kinases
- SH2 domain containing
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene nameLck interacting transmembrane adaptor 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2005-04-25
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data