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Also known as
Lymphoid Enhancer Factor 1
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Other names
lymphoid enhancer-binding factor 1 isoform 1; Lymphoid enhancer-binding factor 1; lymphoid enhancer-binding factor 1; TCF1-alpha; T cell-specific transcription factor 1-alpha; lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
LEF1; LEF1; LEF-1; TCF10; TCF7L3; TCF1ALPHA; LEF-1; TCF1-alpha
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Gene name synonims
LEF1; LEF1; LEF-1; TCF10; TCF7L3; TCF1ALPHA; LEF-1; TCF1-alpha
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Other gene names
LEF1; LEF1; LEF-1; TCF10; TCF7L3; TCF1ALPHA; LEF-1; TCF1-alpha
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
6D538
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Host organism
Mouse (Mus musculus)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes human and mouse LEF. Does not cross-react with T cell factor 3 (TCF3) or T cell factor 4 (TCF4). Detects a band of ~68kDa by Western blot.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Highly Purified by size exclusion chromatography.
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Form Appearance
Supplied as a liquid in PBS, 0.1% sodium azide, PEG and sucrose, before the addiition of glycerol to 40%.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Long-term storage should be done after aliquoting the antibody and freezing the aliqotes at -20 degrees Celsius. Under these conditions the aliquots will be stable for at least one year if kept at -20 °C. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer.
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Tested applications
Western Blot (WB), Immunocytochemistry (ICC)
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Description
The Lymphoid Enhancer Factor 1 (LEF1, Lymphoid Enhancer-binding Factor 1, DKFZp586H0919, HGNC:6551, T Specific Transcription Factor 1 alpha, TCF1 alpha) Antibody is a α- or alpha protein sometimes glycoprotein present in blood. For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
LEF1-AS1, LEF1, HNF1A
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Short name
Lymphoid Enhancer Factor 1 (LEF1, Lymphoid Enhancer-binding Factor 1, DKFZp586H0919, HGNC:6551, T Specific Transcription Factor 1 alpha, TCF1 alpha) Antibody
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Technique
Antibody, antibodies against human proteins, antibodies for
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Alternative name
Anti-Lymphoid Enhancer Factor 1 (LEF1, Lymphoid Enhancer-binding Factor 1, DKFZp586H0919, HGNC:6551, T cell Specific Transcription Factor 1 alpha, TCF1 alpha)
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Alternative technique
antibodies
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Alternative to gene target
lymphoid enhancer-binding factor 1, LEF1 and IDBG-33529 and ENSG00000138795 and 51176, DNA binding, nuclei, Lef1 and IDBG-194292 and ENSMUSG00000027985 and 16842, LEF1 and IDBG-640560 and ENSBTAG00000006844 and 535399
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Tissue
cell
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