TPO, rat recombinant

• Catalog number
  7221-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF
• Alternative_names
  Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF
• Description
  Stimulates the proliferation and maturation of megakaryocytes
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 0.1 ng/ug of protein (
• Activity Specifications test method
  Determined by its ability to stimulate the proliferation of human MO7e cells. The expected ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 10^6 units/mg.
• Biological activity
  Determined by its ability to stimulate the proliferation of human MO7e cells. The expected ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 10^6 units/mg.
• Molecular Weight
  18.7 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Sterile filtered through a 0.2 micron filter. Lyophilized from Sodium Citrate, pH 4.5
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Background Information
  TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant rat TPO is a fully biologically active 174 amino acid polypeptide (18.7 kDa), which contains the erythropoietin-like domain of the full length TPO protein.
• Amino acid sequence
  SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  TPO  
• Gene symbol
  TPO, THPO
• Short name
  TPO, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  Rat
• Species
  Rat, Rats
• Alternative name
  TPO, rat Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:12015
• Gene
  TPO
• Long gene name
  thyroid peroxidase
• Synonyms
  • TPX
• Locus
  2p25.3
• Discovery year
  1986-01-01
• Entrez gene record
  7173
• RefSeq identity
  • NM_000547
• Classification
  • Sushi domain containing
• VEGA ID
  OTTHUMG00000090271

Gene info

• Identity
  HGNC:11795
• Gene
  THPO
• Long gene name
  thrombopoietin
• Synonyms gene
  • MGDF
• Synonyms
  • TPO
  • MPLLG
• Synonyms name
  • prepro-thrombopoietin
  • megakaryocyte stimulating factor
  • myeloproliferative leukemia virus oncogene ligand
  • megakaryocyte growth and development factor
  • MPL ligand
  • megakaryocyte colony-stimulating factor
  • c-mpl ligand
  • thrombopoietin nirs variant 1
• Locus
  3q27.1
• Discovery year
  1994-11-04
• Entrez gene record
  7066
• Pubmed identfication
  • 8202154
• RefSeq identity
  • NM_000460
• Classification
  • Receptor ligands
• VEGA ID
  OTTHUMG00000156745
• Locus Specific Databases
  • LRG_580

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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