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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
Recombinant human Factor VIIa (NovoSeven ®, Novo Nordisk A/S Denmark)
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified total IgG
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How to reconstitute
For reconstitution add 50 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them, to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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Verified applications
prothrombin assay with human plasma (PT), thrombin generation assay, inhibition assay with Factor VIIa with TF and a chromogenic substrate, indirect ELISA (I-ELISA)
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Connected products
AS00 001 | FVIIa | Coagulation factor VIIa (50 µg)IMS01-015-308 | F8 | Coagulation factor VIIIIMS01-118-307 | F7 | Coagulation factor VIIIMS01-137-306 | F12 | Coagulation factor XII Secondary antibodies
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Recommended dilutions for use
1: 15 000 (I-ELISA), 14 µg/ml (IL)
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Molecular weight expected аpparent
52 kDa (this is MW of Factor VIIa)
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Verified reactivity
human
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Possible reactivity
primates
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This antibody is purified using Protein G, to obtain a total IgG fraction.
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References
Lopez-Vilchez et al. (2009). Traffic of rFVIIa through Endothelial Cells and Redistribution into Subendothelium: Implications for a Prolonged Hemostatic Effect. Journal of Coagulation Disorders, October 1: (1). (immunolocalization)
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Scientific context
Factor VIIa (FVIIa) is a key serine protease involved in the initiation of the coagulation cascade. FVIIa requires tissue factor (TF), a membrane bound protein, as an essential cofactor for maximal activity towards its biological substrates Factor X, Factor IX and Factor VII (FVII).
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Notes
no significant difference in binding to Factor VII and Factor VIIa
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Protein number
Q9FI56 , Q9SXJ7
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TAIR number
Refer to NCBI
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Description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.