Ready-to-Use Ni QR Agarose Beads Buffer Kit

• Catalog number
  K6563-3
• Price
  Please ask
• Size
  3 components

• Description
  A ready to use set of loading, elution and extraction buffers to use with the Ni QR agarose beads. Basic research of the physico-chemical properties as well as the activity of proteins is impossible without their isolation and purification. Majority of protein studies are carried out on tagged recombinant proteins expressed in various host organisms. Over 50% of these are expressed as fusions with poly-histidine purification tags. The small size and the mild conditions utilized during purification as well as the low cost of purification made this type of fusion the most popular (and in many cases, the first tag of choice). Ready-to-Use Ni QR Agarose Beads Buffer Kit works seamlessly with Hi-Bind™ Ni QR Agarose Beads (Cat. # 6562) or equivalent from the isolation of target to obtaining highly purified and biologically active protein. These Buffers & Extraction Reagent deliver efficient extraction of target protein (as high as 96% efficiency of SDS boiling method) while preserving its integrity. The buffer components are designed to minimize the metal ion leaching while preserving the biological activity of precious target proteins. This kit is sufficient for purification of up to 300 mg of target protein using 5 ml of Hi-Bind™ Ni QR Agarose Beads (Cat. # K6562) or equivalent under batch/gravity flow conditions.
• Product Highlights
  • Contents: Ni QR Agarose Loading Buffer, Elution Buffer & Extraction Reagent • Sufficient for purification of up to 300 mg of target protein using 5 ml of Hi-Bind™ Ni QR Agarose Beads (Cat. # K6562) or equivalent under batch/gravity flow conditions. • Applications: - Purification of native and recombinant proteins and peptides that have an affinity for metal ions - For purifications under native conditions
• Storage Temp
  +4°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Datasheet
• Notes
  For research use only. Not to be used for human or animal treatment or consumption.
• Properties
  The purest agarose was used in the production of - - Ni QR Agarose Kit by Biovision.
• Conjugation
  Agarose
• Additional description
  Buffering solutions are useful to keep the pH range sable when using this reagent of Biovision.

• Gene target
  Kit  
• Short name
  - - Ni QR Agarose Kit
• Technique
  Agarose, agaroses, All kind of bead conjugates are available, magnetic, borosilicate, agarose protein A. magnetic beads can be isolated by a macs type magnet.
• Label
  Agarose
• Alternative name
  Ready-to-Use Ni QR molecular sieve particles solution reagent
• Alternative technique
  separation, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Electrophoresis, Agar Gel
• Concept
  Scope note: Electrophoresis in which agar or agarose gel is used as the diffusion medium.
• Tree numbers
  • E05.196.401.153
  • E05.301.300.100
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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