PRDX3 Antibody (Peroxiredoxin 3)
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Catalog number
R31762
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Price
Please ask
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Size
0.1mg
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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
PRDX3 (Peroxiredoxin 3)
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, IHC-P
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Recommended dilutions
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
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Notes
The stated application concentrations are suggested starting amounts. Titration of the PRDX3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This PRDX3 antibodyis to be used only for research purposes and not for diagnostics..
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Gene ID
10935
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Purity
Antigen affinity
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Description
Peroxiredoxin 3, also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human protein shares 86% amino acid sequence similarity with mouse, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23. The PRDX3 gene is mapped on 10q26.11. Expression of Peroxiredoxin 3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that Peroxiredoxin 3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that PRDX3 is a MYC target gene that is required to maintain normal mitochondrial function.
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Immunogen
Human partial recombinant protein (AA 110-256) was used as the immunogen for this PRDX3 antibody.
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Storage
After reconstitution, the PRDX3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
PRDX3
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Short name
Anti-PRDX3 (Peroxiredoxin 3)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
Antibodies to PRDX3 (Peroxiredoxin 3)
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Alternative technique
antibodies
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Alternative to gene target
peroxiredoxin 3, AOP-1 and AOP1 and HBC189 and MER5 and PRO1748 and prx-III and SP-22, PRDX3 and IDBG-91076 and ENSG00000165672 and 10935, peroxiredoxin activity, Cytoplasm, Prdx3 and IDBG-181859 and ENSMUSG00000024997 and 11757, PRDX3 and IDBG-640354 and ENSBTAG00000008731 and 281998
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Gene info
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Identity
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Gene
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Long gene name
peroxiredoxin 3
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Synonyms gene
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Synonyms gene name
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Synonyms
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GenBank acession
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Locus
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Discovery year
1999-08-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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