EGF, rat recombinant
-
Catalog number4024-1000
-
PricePlease ask
-
Size1 mg
-
-
SynonymsUrogastrone, URG, EGF, epidermal growth factor
-
Alternative_namesUrogastrone, URG, EGF, epidermal growth factor
-
DescriptionA growth factor stimulating cell growth, proliferation, and differentiation by binding to EGFR
-
RecombinantYes
-
SourceE. coli
-
Purity by SDS PAGE≥98%
-
AssaySDS-PAGE
-
Purity≥98%
-
Biological activityThe ED₅₀ as calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of > 1 x 10⁷units/mg.
-
Molecular Weight6.151 kDa
-
Storage Temp-20°C
-
ShippingGel pack
-
Shelf Life12 months
-
AppearanceLyophilized protein
-
Physical form descriptionLyophilized from PBS, pH 7.4
-
Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile H₂O to a concentration ≥ 100 µg/ml. This solution can then be diluted into other aqueous buffer.
-
Background InformationEpidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Epidermal Growth Factor Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6151 Dalton. The Rat EGF is purified by proprietary chromatographic techniques.
-
Amino acid sequenceNSNTGCPPSYDGYCLNGGVCMYVESVDRYVCNCVIGYIGERCQHRDLRWWKLR
-
HandlingCentrifuge the vial prior to opening.
-
UsageFor Research Use Only! Not to be used in humans
-
AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
Latin nameRattus norvegicus
-
Additional sourceRecombinants or rec. proteins
-
Grouprecombinants
-
Gene target
-
Gene symbolEGF
-
Short nameEGF, recombinant
-
TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
HostRat
-
SpeciesRat, Rats
-
Alternative nameepidermal growth factor, rat Rec.
-
Alternative techniquerec
-
Alternative to gene targetepidermal growth factor, HOMG4 and URG, EGF and IDBG-34100 and ENSG00000138798 and 1950, transmembrane receptor protein tyrosine kinase activator activity, Extracellular, Egf and IDBG-193432 and ENSMUSG00000028017 and 13645
-
Gene info
-
Identity
-
Gene
-
Long gene nameepidermal growth factor
-
Synonyms gene name
- epidermal growth factor (beta-urogastrone)
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data