Recombinant Human Osteonectin/SPARC/BM-40 Protein(C-6His)

• Catalog number
  PKSH032839-10µg
• Price
  Please ask
• Size
  10µg

• Synonym
  SPARC; Basement-Membrane Protein 40; BM-40; Osteonectin; ON; Secreted Protein Acidic and Rich in Cysteine; SPARC; ON
• Activity
  NA
• Sequence
  Ala18-Ile303
• Fusion tag
  C-6His
• Accession
  P09486
• Expressed Host
  Human cells
• Shipping
  The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
• Purity
  ＞95% as determined by reducing SDS-PAGE.
• Endotoxin
  ＜1.0 EU per µg as determined by LAL test.
• Stability and Storage
  Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
• Mol Mass
  33.7 kDa
• AP Mol Mass
  36 kDa
• Formulation
  Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Background
  Secreted Protein Acidic and Rich in Cysteine (SPARC) is a secreted, evolutionarily conserved collagen-binding glycoprotein and belongs to the SPARC family. SPARC has 286 amino acids and contains an EF-hand in C-termina domain, a follistatin-like domain with Kazal-like sequences. There are two calcium binding sites, one binds 5 - 8 Ca2+ with a low affinity and other on an EF-hand loop that binds a Ca2+ ion with a high affinity. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC regulate cell growth through interactions with the extracellular matrix (ECM) and cytokines. SPARC bind to numerous proteins of the ECM, affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC also binds several types of collagen, albumin, thrombospondin, PDGF and cell membranes.
• Technical data
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Osteonectin/SPARC/BM-40   Protein   C-6His  
• Gene symbol
  SPARC, SNORD115-40, CLMAT3, IGHV7-40, IGKV2D-40, PPID, IGKV2-40, IGLV1-40, IGHVII-40-1, PHF21A
• Short name
  Recombinant Osteonectin/SPARC/BM-40 Protein(C-6His)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Osteonectin/secreted protein, acidic, cysteine-rich (osteonectin)/BM-40 Protein(C-6His)
• Alternative technique
  rec
• Alternative to gene target
  secreted protein, acidic, cysteine-rich (osteonectin), ON, SPARC and IDBG-54562 and ENSG00000113140 and 6678, extracellular matrix binding, nuclei, Sparc and IDBG-175174 and ENSMUSG00000018593 and 20692, SPARC and IDBG-646341 and ENSBTAG00000014835 and 282077

Gene info

• Identity
  HGNC:11219
• Gene
  SPARC
• Long gene name
  secreted protein acidic and cysteine rich
• Synonyms gene
  • ON
• Synonyms gene name
  • osteonectin
• Synonyms
  • ONT
  • BM-40
• Synonyms name
  • cysteine-rich protein
  • secreted protein acidic and rich in cysteine
• Locus
  5q33.1
• Discovery year
  2001-06-22
• Entrez gene record
  6678
• Pubmed identfication
  • 2838412
  • 3410046
• RefSeq identity
  • NM_003118
• Classification
  • SPARC family
• VEGA ID
  OTTHUMG00000130122

Gene info

• Identity
  HGNC:33059
• Gene
  SNORD115-40
• Long gene name
  small nucleolar RNA, C/D box 115-40
• Synonyms
  • HBII-52-40
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033814
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003355.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:52287
• Gene
  CLMAT3
• Long gene name
  colorectal liver metastasis associated transcript 3
• Synonyms
  • SPARC-AS1
• Synonyms name
  • SPARC antisense RNA 1
• Locus
  5q33.1
• Discovery year
  2017-06-15
• Entrez gene record
  101927096
• Pubmed identfication
  • 26050227
• RefSeq identity
  • NR_109873
• Classification
  • Long non-coding RNAs with non-systematic symbols

Gene info

• Identity
  HGNC:5666
• Gene
  IGHV7-40
• Long gene name
  immunoglobulin heavy variable 7-40 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 7-40
  • immunoglobulin heavy variable 7-40 pseudogene
• GenBank acession
  • M99644
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28381
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152088

Gene info

• Identity
  HGNC:5804
• Gene
  IGKV2D-40
• Long gene name
  immunoglobulin kappa variable 2D-40
• GenBank acession
  • X59311
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28878
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000159963

Gene info

• Identity
  HGNC:9257
• Gene
  PPID
• Long gene name
  peptidylprolyl isomerase D
• Synonyms gene name
  • peptidylprolyl isomerase D (cyclophilin D)
• Synonyms
  • CYP-40
  • CypD
• Synonyms name
  • cyclophilin 40
• Locus
  4q32.1
• Discovery year
  1995-08-23
• Entrez gene record
  5481
• Pubmed identfication
  • 8509368
• RefSeq identity
  • NM_005038
• Classification
  • Cyclophilin peptidylprolyl isomerases
  • Tetratricopeptide repeat domain containing
• VEGA ID
  OTTHUMG00000161927

Gene info

• Identity
  HGNC:5789
• Gene
  IGKV2-40
• Long gene name
  immunoglobulin kappa variable 2-40
• GenBank acession
  • X59314
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28916
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000188265

Gene info

• Identity
  HGNC:5877
• Gene
  IGLV1-40
• Long gene name
  immunoglobulin lambda variable 1-40
• GenBank acession
  • M94116
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28825
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151072

Gene info

• Identity
  HGNC:5679
• Gene
  IGHVII-40-1
• Long gene name
  immunoglobulin heavy variable (II)-40-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-40-1
  • immunoglobulin heavy variable (II)-40-1 pseudogene
• Synonyms
  • IGHV(II)-40-1
• GenBank acession
  • AB019438
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28368
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152098

Gene info

• Identity
  HGNC:24156
• Gene
  PHF21A
• Long gene name
  PHD finger protein 21A
• Synonyms
  • BHC80
  • KIAA1696
  • BM-006
• GenBank acession
  • AL359593
• Locus
  11p11.2
• Discovery year
  2005-02-07
• Entrez gene record
  51317
• Pubmed identfication
  • 11214970
  • 12032298
• RefSeq identity
  • NM_016621
• Classification
  • PHD finger proteins
• VEGA ID
  OTTHUMG00000167038

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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