p70 S6 Kinase Antibody
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Catalog number3485-100
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PricePlease ask
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Size100 µg
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Antibody Targetp70 S6 Kinase
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Target Alternative NameRPS6KB1 , p70S6k , p70 , S6K , p70(S6K)-alpha , P70S6K , p70(S6K)-alpha , p70-alpha
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic peptide near the C-terminus of human p70 S6 kinase
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AccessionQ9UBS0
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Gene Id6199
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µg (0.5 mg/ml) antigen affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.
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PurificationAffinity purified
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ApplicationWestern blot, Immunoprecipitation, Immunohistochemistry
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Application And UsagesWestern blotting (1 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes p70 S6 kinase of human, mouse, and rat origins. Reactivity to other species has not been tested.
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Descriptionp70 S6 kinase and p90 Rsk kinase are the two distinct 40S ribosomal protein S6 Ser/Thr kinases present in somatic animal cells. p90 Rsk kinase is maximally activated within minutes of addition of growth factors or phorbol ester to cultured cells followed by activation of p70 S6 kinase. Both enzymes are regulated by serine/threonine phosphorylation, sμggesting that specific kinases may exist upstream in the signaling pathways that regulate these kinases.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolRPS6KB1, RPS6KB2
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Short namep70 S6 Kinase Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative namep70 S6 phosphorylation catalyst (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameribosomal protein S6 kinase B1
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Synonyms gene
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Synonyms gene name
- ribosomal protein S6 kinase, 70kD, polypeptide 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-07-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- AGC family kinases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameribosomal protein S6 kinase B2
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Synonyms gene name
- ribosomal protein S6 kinase, 70kD, polypeptide 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-07-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- AGC family kinases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data