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Alternative name 1
FAS Antigen, Intracellular fragment, Clone: 5F-9, Mab anti-; IP/flow/WB/ELISA/prevents apoptosis induced
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Alternative name 2
FAS Antigen, Intracellular fragment, Clone: 5F-9, Mab antibody-; IP/flow/WB/ELISA/prevents apoptosis induced
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Alternative name 3
FAS Antigen, Intracellular fragment, Clone: 5F-9, Mab antibody-; IP/flow/WB/ELISA/prevents apoptosis induced
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Alternative name 4
anti-FAS Antigen Intracellular fragment Clone: 5F-9 IP/flow///prevents apoptosis induced
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Alternative name 5
FAS Antigen Intracellular fragment Clone: 5F-9 IP/flow///prevents apoptosis induced
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Clonality
Monoclonal Antibody
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Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Additional description
Antigens are peptides or recombinant or native dependent on the production method. This 1 is suited for programmed cell-death studies. Induced protein genes, factors or kinases, increase the production of (an enzyme or other protein) at the level of genetic transcription.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
FAS-AS1, FASN, RNU6-9, PIRC47, PIRC38, PIRC43, PIRC45
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Short name
FAS Antigen, Intracellular , Clone: 5F-9, Mouse Monoclonal antibody-; IP/ / /ELISA/prevents apoptosis
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Technique
Mouse monoclonal, intracellular, apoptosis, Antibody, antigen, ELISA, Mouse, Flow, IP, WB, antibodies against human proteins, antibodies for, antigenes, apoptosis pathway, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. Immunoprecipitation, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals, Western Blot on a membrane
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Host
mouse monoclonal, antigen assay ELISA kit 1 Kit (96 Wells), Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
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Species
Mouse, Mouses
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Alternative name
Fas (tumor necrosis factor receptor superfamily, member 6) protein, Intracellular fragment, clonality: 5F-9, Mouse monoclonal (antibody to-)-; IP/flow/Western Blot/Enzyme-linked immunosorbent assay/prevents programmed cellular destruction induced
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Alternative technique
celldeath, antibodies, antigenes, murine, elisas
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Alternative to gene target
Fas (TNF receptor superfamily, member 6), ALPS1A and APO-1 and APT1 and CD95 and FAS1 and FASTM and TNFRSF6, FAS and IDBG-81669 and ENSG00000026103 and 355, identical protein binding, nuclei, Fas and IDBG-159577 and ENSMUSG00000024778 and 14102, FAS and IDBG-637356 and ENSBTAG00000010785 and 282488
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