Cultrex Laminin (mouse)
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Catalog number3400-010-01
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PricePlease ask
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Size1 mg
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Keep at-80° C
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Assay performanceCultrexes are the best 3D matrixes for cell cultures known as matrigel or cultrex. Basal membrane extracts higher the differentiation capacity or from basal membrane over what can be achieved by 2D monolayer cultures from epithelial cells. You can use cultrex for high differentiation in a 3D matrix as Basement Membrane Extracts (BME), extracellular matrix from lumen epithelial or endothelial cells. BME, collagen I, collagen IV, laminin I, fibronectin and vitronectin are pathogen tested and suitable for 3d and pluripotent stem cell culture. Cultrex Basement Membrane and the PathClear Protocol is supplied in a liquid form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor at a concentration of 13 - 17 mg/ml from Murine Engelbreth-Holm-Swarm (EHS) tumor with Dulbecco’s Modified Eagle’s medium without phenol red and with 11 ug/ml gentamicin sulfate with cultrex BME for thin Layer Method (non-gelling).
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More adviceCultrex 3D cell culture for cancer studies cell invasion and 3 dimensional cell expansion up to 2/3 conflunecy
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TestThe gelatin protein mixture of Trevigen is called Cultrex and it is a matrigel like secreted supernatant of the EHS mouse sarcoma cell line. It is a 3D structure similar to Tissues and cells will to adhere to this basement membrane extract BME very well. Cultrexes are the best medium for 3D cell culture studies. They can be used as a metastasis model or an iPSC model (induced pluripotent stem cell). The cultrex medium is useful for cell migration studies using the Pathclear endotoxin free and sterile basement membrane extract. Enghelbreth Holm Swarm sarcoma cells are sold by Corning. A collagen, laminin, fibronectin and vitrobectin Cultrexes are available from Trevigen. Cultrex® Human has been tested for the viruses should be handled in a BSL2 ( biosafety level 2 ) environment. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolLAMB2, LAMA5, LAMA4
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Short nameCultrex Laminin (mouse)
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TechniqueCultrex, Mouse, cultrex, mouses
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Hostmouse
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SpeciesMouse, Mouses
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Alternative nameCultrex Laminin (mouse)
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Alternative techniquecultrexes, murine
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Gene info
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Identity
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Gene
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Long gene namelaminin subunit beta 2
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Synonyms gene
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Synonyms gene name
- laminin, beta 2 (laminin S)
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Synonyms
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Synonyms name
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Locus
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Discovery year1992-05-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Laminin subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelaminin subunit alpha 5
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Synonyms gene name
- laminin, alpha 5
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GenBank acession
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Locus
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Discovery year1997-10-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Laminin subunits
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelaminin subunit alpha 4
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Synonyms gene name
- laminin, alpha 4
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Synonyms
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Locus
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Discovery year1993-07-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Laminin subunits
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VEGA ID
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Locus Specific Databases