PDGF-AA, human recombinant
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Catalog number4482-50
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PricePlease ask
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Size50 ug
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SynonymsGlioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.
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Alternative_namesGlioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1, Alpha platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor
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DescriptionA growth factor that regulates cell growth and division
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Purity≥98%
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Biological activityThe ED₅₀ as determined by the dose-dependent stimulation of thymidine uptake by Balb-c 3T3 cells is < 1 ng/ml, corresponding to a specific activity of >1 x 10⁶ units/mg.
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Results< 1 ng/ml
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Molecular Weight28.5 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionLyophilized with no additives
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Reconstitution InstructionsReconstitution in 10 mM acetic acid to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
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Background InformationPlatelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AA is a 28.5 kDa A chain homodimer protein containing 250 amino acid residues.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolPDGFA, TEAD1
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Short namePDGF-AA, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative namePDGF-AA, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameplatelet derived growth factor subunit A
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Synonyms gene name
- platelet-derived growth factor alpha polypeptide
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Synonyms
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Synonyms name
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTEA domain transcription factor 1
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Synonyms gene
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Synonyms gene name
- atrophia areata, peripapillary chorioretinal degeneration
- TEA domain family member 1 (SV40 transcriptional enhancer factor)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1994-09-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- TEA domain transcription factors
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data