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Available ordering format
Lyophilized in phosphate buffer saline
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Immunogen
native peroxidase isolated and purified from horseradish
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified IgG
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How to reconstitute
For reconstitution please add 0.5 ml of sterile destilled water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
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Connected products
AS09 548| anti-HRP horseradishPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 1000 - 1: 10 000 for techniques listed above
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Molecular weight expected аpparent
40 kDa
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Verified reactivity
Armoracia rusticana
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Possible reactivity
Armoracia rusticana
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
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References
to be added when available
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Scientific context
Horseradish peroxidase removes hydrogen peroxide, acts in oxidation of toxic reductants, biosynthesis and degradation of lignin, response to environmental stresses such as wounding, pathogen attack and oxidative stress.
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Notes
antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Test
A high affinity purification column was use to purify , horseradish , by agisera by chromatographic size exclusion.
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Description
1 of protein A or G purified by agisera will give more specificity than crude serum.
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated
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Conjugate
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of
ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
