Mouse Tissue factor/F3 PicoKine ELISA Kit

  • Catalog number
    EK1367
  • Price
    Please ask
  • Size
    1x96 well plate
  • ELISA s application
    For quantitative measurement of mouse Tissue factor in cell culture supernatant, serum, plasma heparin, EDTA, citrate and cell lysate.
  • Gene tested
    murine Tissue factor
  • ELISA standard curve
    Concentration(pg/ml) + 0 + 15.6 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 O.D. + 0.012 + 0.026 + 0.050 + 0.091 + 0.197 + 0.507 + 1.043 + 2.294
  • ELISA kit detection limits
    15.6pg/ml to 1000pg/ml
  • ELISA s sensitivity
    < 10pg/ml
  • Target of this ELISA
    Natural and recombinant mouse Tissue factor
  • Cross reaction
    the ELISA does not cross react with any other proteins except from closely related species
  • ELISA s precision parameters
    intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);113;327;603;120;315;592 ELISA standard deviations 4.41;14.4;30.8;5.64;18.3;37.3 CV(%);3.9;4.4;5.1;4.7;5.8;6.3
  • ELISA kit description
    GENTAUR's mouse Tissue factor ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for Tissue factor was used as capure antibodies on the ELISA test plate. The internal standards (NSO, A29-E251) and your samples are measured with a biotin conjugated polyclonal from goat specific for Tissue factor will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse Tissue factor in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
  • Detector and capure antibodies
    A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
  • The ELISA contains
    Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse Tissue factor antibodies;1 ST0000-10;Lyophilized recombinant mouse Tissue factor standard;10ng/tube×2 AR1107;Biotinylated anti- mouse Tissue factor antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
  • Anti coagulation
    heparin, EDTA or citrate
  • How the elisa works
    Sample the amount of 100ul/well of the standard solution at 1000 pg per ml, 500, 25, 125, 62.5, 31.25, 15.6 pg/ml mouse Tissue factor standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum, plasma(heparin, EDTA, citrate) or cell lysate to each empty well.. See “Sample dilutions Guideline” above for details. It is recommended that each mouse Tissue factor standard solution and each sample be measured in duplicate.
  • Minutes of TMB incubation
    15-20
  • More gene info
    Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. Tissue factor gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.
  • Antigen
    NSO, A29-E251
  • Keep at
    Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
  • Uniprot
    Tissue factor
  • Gene name
    coagulation factor III (thromboplastin, tissue factor)
  • Alternative
    CD142;Coagulation factor III;F3;TF;TF_HUMAN;TFA;Thromboplastin;Tissue factor
  • Gen protein identity
    P20352
  • Entrez nr
    14066
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Description
    Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. 6
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    F3
  • Short name
    Mouse Tissue factor/F3 PicoKine ELISA Kit
  • Technique
    ELISA kit, tissue, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses, tissues
  • Host
    mouse, antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Tissue factor/coagulation factor III (thromboplastin, tissue factor) PicoKine Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    tissues, murine, elisas, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Tissue
    tissue
Gene info
  • Identity
  • Gene
  • Long gene name
    solute carrier family 35 member F3
  • Synonyms gene name
    • solute carrier family 35, member F3
  • Synonyms
  • Locus
  • Discovery year
    2003-11-25
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Solute carriers
    • MicroRNA protein coding host genes
  • VEGA ID
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The technique of using FIXATIVES in the preparation of cytologic, histologic, or pathologic specimens for the purpose of maintaining the existing form and structure of all the constituent elements.
  • Tree numbers
    • E01.370.225.500.620.760.720
    • E01.370.225.750.600.760.720
    • E05.200.500.620.760.720
    • E05.200.750.600.760.720
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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