Human IL-1 beta PicoKine ELISA Kit

  • Catalog number
    EK0392
  • Price
    Please ask
  • Size
    1x96 well plate
  • ELISA s application
    For quantitative measurement of human IL-1 beta in cell culture supernatant, serum and plasma heparin, EDTA, citrate .
  • Gene tested
    Human IL-1 beta
  • ELISA standard curve
    Concentration(pg/ml) + 0 + 3.9 + 7.8 + 15.6 + 31.2 + 62.5 + 125 + 250 O.D. + 0.003 + 0.086 + 0.161 + 0.292 + 0.557 + 1.029 + 1.617 + 2.222
  • ELISA kit detection limits
    3.9pg/ml to 250pg/ml
  • ELISA s sensitivity
    < 0.15pg/ml
  • Target of this ELISA
    Natural and recombinant human IL-1 beta
  • Cross reaction
    the ELISA does not cross react with any other proteins except from closely related species
  • ELISA s precision parameters
    intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);19;41.2;116;27.5;61.3;179.1 ELISA standard deviations 1.0;2.6;6.5;1.6;4.1;12.7 CV(%);5.3;6.3;5.6;5.8;6.7;7.1
  • ELISA kit description
    GENTAUR's human IL-1 beta ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for IL-1 beta has been precoated onto 96-well plates. Standards (E.coli,A117-S269) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL-1 beta will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human IL-1 beta in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
  • Detector and capure antibodies
    a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
  • The ELISA contains
    Catalog number;Description;Quantity -;96-well plate precoated with anti- human IL-1 beta antibodies;1 ST0000-1;Lyophilized recombinant human IL-1 beta standard;1ng/tubex2 AR1107;Biotinylated anti- human IL-1 beta antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
  • Anti coagulation
    heparin, EDTA or citrate
  • How the elisa works
    Sample the amount of 100ul/well of the standard solution at 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 16.5pg/ml, 7.8pg/ml, 3.9pg/ml human IL-1 betastandard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each human IL-1 betastandard solution and each sample is measured in duplicate.
  • Minutes of TMB incubation
    15-20
  • More gene info
    Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
  • Antigen
    E.coli,A117-S269
  • Keep at
    Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
  • Uniprot
    Interleukin-1 beta
  • Gene name
    interleukin 1, beta
  • Alternative
    Catabolin;_x000D_Hematopoietin 1;_x000D__x000D__x000D_IFN beta inducing factor;_x000D_IL 1;_x000D_IL 1 beta;_x000D_IL 1B;_x000D_IL-1 beta;_x000D_IL1;_x000D_IL1 BETA;_x000D_IL1B;_x000D_IL1B_HUMAN;_x000D_IL1F2;_x000D_Interleukin 1 beta;_x000D_Interleukin 1 beta precursor;_x000D_Interleukin-1 beta;_x000D_LAF;_x000D_OAF;_x000D_Osteoclast activating factor;_x000D_Preinterleukin beta;_x000D_Pro interleukin 1 beta
  • Gen protein identity
    P01584
  • Entrez nr
    3553
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Gene
    The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
  • Gene target
    IL-1   beta   PicoKine  
  • Gene symbol
    IL1B
  • Short name
    IL-1 beta PicoKine ELISA Kit
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Human, Humans
  • Alternative name
    H. sapiens Interleukin-1 b PicoKine Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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