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Synonyms
ICOS, CD278, AILIM, Inducible T-cell costimulator
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Alternative_names
ICOS, CD278, AILIM, Inducible T-cell costimulator
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Description
Inducible T-cell costimulator
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Recombinant
True
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Source
HEK 293 cells
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Purity by SDS PAGE
>90%
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Assay
SDS-PAGE
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Endotoxin Level
Less than 1.0 EU per ug by the LAL method.
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Biological activity
Immobilized Human B7-H2 at 1 ug/mL (100 uL/well) can bind Mouse ICOS with a linear range of 0.01-0.2ng/mL
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Molecular Weight
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.7 kDa. The protein migrates as 22-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Lyophilized
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Reconstitution Instructions
Reconstitute in sterile deionized water to the desired protein concentration.
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Background Information
Inducible T-cell costimulator (ICOS) is also known as Activation-inducible lymphocyte immunomediatory molecule (AILIM), CD278, which belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. ICOS enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. CD278 / ICOS prevents the apoptosis of pre-activated T-cells and also plays a critical role in CD40-mediated class switching of immunoglobin isotypes.
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Amino acid sequence
Glu 21 - Leu 144
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Handling
Centrifuge the vial prior to opening.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
ICOS, ICOSLG
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Short name
CellExp™ ICOS / CD278, Mouse Recombinant
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Technique
Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Host
mouse
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Species
Humans, Mouses
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Alternative name
H. sapiens CellExp™ inducible T-cell co-stimulator / CD278, Mouse Rec.
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Alternative technique
rec, murine
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Alternative to gene target
inducible T-cell co-stimulator, ICOS and IDBG-79246 and ENSG00000163600 and 29851, protein binding, Extracellular, Icos and IDBG-159737 and ENSMUSG00000026009 and 54167, ICOS and IDBG-643378 and ENSBTAG00000021596 and 507026
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