Rabbit Anti Human ATG10 Antibody-FITC
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Catalog numberCSB-PA861988LC01HU_100ug
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PricePlease ask
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Size100ug
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Long nameRabbit Antibody against Human ATG10, FITC
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Synonym nameATG10 Human Antibody
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Species reactivityHuman
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Tested applications recommended dilutionsELISA
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ConjugationFITC
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Antibody isotypeIgG
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UniProt IDQ9H0Y0
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FormatLiquid, diluted in buffer
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Buffer compositionPreservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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Purity>95%
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Purification methodProtein G purified
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Antibody categoryPolyclonal Antibodies
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Immunogen typeRecombinant human Ubiquitin-like-conjugating enzyme ATG10 protein (1-50AA)
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Immunogen speciesHuman
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AliasUbiquitin-like-conjugating enzyme ATG10,Autophagy-related protein 10,APG10-like,ATG10,APG10L,PP12616
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BackgroundE2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis.
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ClonalityPolyclonal antibody
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Shipping and storageATG10 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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PropertiesIf you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cusabio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cusabio FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional conjugationAnti-FITC Antibody
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AboutRabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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French translationanticorps
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Gene target
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Gene symbolATG10-AS1, ATG10-IT1, ATG10
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Short nameAnti-ATG10
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Techniqueanti human, Antibody, Rabbit, anti, FITC, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, Fluorescein
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HostRabbit, Rabbits
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LabelFITC
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SpeciesHuman, Humans
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Alternative nameATG10 Antibody,FITC conjugated
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Alternative techniqueantibodies, rabbit-anti, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameATG10 antisense RNA 1
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Synonyms gene name
- ATG10 antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2011-05-19
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameATG10 intronic transcript 1
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Synonyms gene name
- ATG10 intronic transcript 1 (non-protein coding)
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Locus
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Discovery year2011-05-19
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Classification
- Intronic transcripts
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameautophagy related 10
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Synonyms gene
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Synonyms gene name
- APG10 autophagy 10-like (S. cerevisiae)
- ATG10 autophagy related 10 homolog (S. cerevisiae)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-01-28
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Entrez gene record
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RefSeq identity
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Classification
- Autophagy related
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data