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Immunogen
Synthetic peptide from the N-terminal of Human ATG4B (aa. 62-73)
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Antibody s target
Human ATG4B
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Antibody s full description
Rabbit Anti-Human ATG4B Polyclonal
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Primary research fields
Cancer, Autophagy, Cardiovascular System, Heart, Cell Signaling
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
MGC1353 Antibody, Autophagy-related cysteine endopeptidase 1 Antibody, ATG4B Antibody, hAPG4B Antibody, AUT-like 1 cysteine endopeptidase Antibody, Cysteine protease ATG4B Antibody, Autophagy-related protein 4 homolog B Antibody, Autophagin-1 Antibody, EC:3.4.22.- Antibody, ATG4B_HUMAN Antibody, APG4B Antibody, APG4 autophagy 4 homolog B Antibody, AUTL1 Antibody, KIAA0943 Antibody, Autophagy related cysteine endopeptidase 1 Antibody, Autophagin 1 Antibody, ATG4 autophagy related 4 homolog B (S. cerevisiae) Antibody, AUT like 1 cysteine endopeptidase Antibody, Autophagy related protein 4 homolog B Antibody,
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Verified applications
WB, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse
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Antibody s recommended dilutions for use
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~50 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-631 was sufficient for detection of ATG4B in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
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Scientific context
Autophagy related 4B, cysteine peptidase (ATG4B or APG4B) is a C-54 cysteine protease that also belongs to the family of autophagin poteins. Specifically it cleaves LC3, and mutations can be associated with strong inhibition of autophagosome formation.
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Bibliography
1. Kabeya Y., et al. (2004) J Cell Sci. 117: 2805-2812. 2. Tanida I., et al. (2004) J Biol Chem. 279: 36268-36276. 3. Hemelaar J., et al. (2003) J Biol Chem. 278: 51841-51850.
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Released date
29/Aug/2016
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NCBI number
NP_037457.3
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Gene number
23192
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Protein number
Q9Y4P1
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4B Polyclonal Antibody (SPC-631). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody (SPC-631) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4B Antibody (D) Composite. | Western blot analysis of Mouse Brain cell lysates showing detection of 44.3 kDa ATG4B protein using Rabbit Anti-ATG4B Polyclonal Antibody (SPC-631). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4B Polyclonal Antibody (SPC-631) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 44.3 kDa. Rabbit Anti-ATG4B Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-631) | Rabbit Anti-ATG4B Antibody used in Western blot (WB) on Brain cell lysates (SPC-631)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus