DCL3, Dicer-like protein 3
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Catalog number
AS12 2103
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Price
Please ask
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Size
200 µg
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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DCL3 sequence, Q9LXW7 , At3g43920
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 200 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS12 2102 | anti-DCL1 | Dicer-like protein 1, rabbit antibodiesAS15 3100 | anti-DCL2 | Endoribonuclease Dicer homolog 2, rabbit antibodiesAS15 3105 | anti-DCL4 | Dicer-like protein 4, rabbit antibodiescollection of antibodies to proteins involved in microRNA metabolismPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 1000 with standard ECL (WB)
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Molecular weight expected аpparent
117 kDa
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Verified reactivity
Arabidopsis thaliana
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Possible reactivity
Arabidopsis thaliana
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No reactivity
Zea mays
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Supplementary information
Recommend protein load is from 40-50 µg/well (using 1.5 mm spacers helps to obtain wider wells)
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References
to be added when available, antibody released in August 2012.
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Scientific context
DCL3 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Synonymes: Endoribonuclease Dicer homolog 3.
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Notes
None
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Protein number
Q9LXW7
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TAIR number
AT3G43920
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Gene target
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Short name
DCL3, Dicer-like protein 3
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Alternative name
DCL3, Dicer-like protein 3
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