Target of this antibody:
Alternative names of antibody target:
T cell antigen receptor, T cell receptor beta, TCRB, TRB
TCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either α and β or γ and δ chains. The vast majority of circulating T cells (95%) express the α/β heterodimer while roμghly 2-5% express the γ/δ heterodimer. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction throμgh the TCR.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 114 of human TCR-β
Concentration of antibody:
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Western blotting (0.5-4 µg/ml). Based on researcher’s feedback, the antibody can also be used in immunoprecipitation (10-20 µg/ml); and Immunohistochemistry (15-25 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes multiple TCR-beta forms including 17 kDa monomer, 34 kDa dimer and the 68 kDa precursor forms. Jurkat cell lysate can be used as a positive control.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.