TCR beta Antibody
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Catalog number5651-30T
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PricePlease ask
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Size30 µg
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Antibody TargetTCRb
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Target Alternative NameT cell antigen receptor, T cell receptor beta, TCRB, TRB
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic peptide surrounding amino acid 114 of human TCR-β
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AccessionP01850
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Gene Id28639
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AppearanceColorless liquid
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FormLiquid
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Concentration0.2 mg/ml
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FormulationIn PBS pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% Proclin.
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PurificationAffinity purified
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ApplicationWestern blot, Immunoprecipitation, Immunohistochemistry
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Application And UsagesWestern blotting (0.5-4 µg/ml). Based on researcher’s feedback, the antibody can also be used in immunoprecipitation (10-20 µg/ml); and Immunohistochemistry (15-25 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes multiple TCR-beta forms including 17 kDa monomer, 34 kDa dimer and the 68 kDa precursor forms. Jurkat cell lysate can be used as a positive control.
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DescriptionTCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either α and β or γ and δ chains. The vast majority of circulating T cells (95%) express the α/β heterodimer while roμghly 2-5% express the γ/δ heterodimer. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction throμgh the TCR.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Short nameTCR beta Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameTCR b (antibody to-)
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data