Anti-Acid Phosphatase (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-6291R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  Acid Phosphatase
• Specificity
  This antibody reacts specifically with Acid Phosphatase
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic peptide derived from human Acid Phosphatase
• Gene ID
  5068
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Phosphatase enzymes catalyse hydrolysis of phosphoric acid esters of various alcohols, e.g. a hexose phosphate, to yield the alcohol and free inorganic phosphate. This may be a way of recycling phosphate in the cell, and the level of phosphate may be elevated under conditions of phosphate starvation (in algae for example). Acid phosphatases have pH optima below 7, whereas alkaline phosphatases are most active above pH 7. Commercial quantities of acid phosphatase are obtained from plant sources e.g. potato and wheat germ, whereas similar quantities of alkaline phosphatase are obtained from animal intestine and bacteria.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  Acid phosphatase 1 soluble; Acid phosphatase of erythrocyte; Adipocyte acid phosphatase; Cytoplasmic phosphotyrosyl protein phosphatase; HAAP; Low molecular weight phosphotyrosine protein phosphatase; PAP1; PAP2; Protein tyrosine phosphatase; PTPase; Purple acid phosphatase; Red cell acid phosphatase 1; PPAC_HUMAN.
• Also known as
  Acid Phosphatase Polyclonal Antibody
• Other name
  Anti-Acid Phosphatase Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  Acid   Phosphatase  
• Gene symbol
  ACP4, ACP1, ACP2
• Short name
  Anti-Acid Phosphatase
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  Acid Phosphatase Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:14376
• Gene
  ACP4
• Long gene name
  acid phosphatase 4
• Synonyms gene
  • ACPT
• Synonyms gene name
  • acid phosphatase, testicular
• Synonyms name
  • testicular acid phosphatase
• GenBank acession
  • AF321918
• Locus
  19q13.33
• Discovery year
  2001-07-13
• Entrez gene record
  93650
• Pubmed identfication
  • 11414767
• RefSeq identity
  • NM_033068
• Classification
  • Acid phosphatases
• VEGA ID
  OTTHUMG00000182961

Gene info

• Identity
  HGNC:122
• Gene
  ACP1
• Long gene name
  acid phosphatase 1
• Synonyms gene name
  • acid phosphatase 1, soluble
• Synonyms
  • HAAP
  • LMW-PTP
  • LMWPTP
• Synonyms name
  • acid phosphatase of erythrocyte
  • low molecular weight phosphotyrosine protein phosphatase
  • red cell acid phosphatase
• GenBank acession
  • M87546
• Locus
  2p25.3
• Discovery year
  1986-01-01
• Entrez gene record
  52
• Pubmed identfication
  • 457131
  • 1587862
  • 8586411
• RefSeq identity
  • NM_004300
• Classification
  • Acid phosphatases
  • Class II Cys-based phosphatases
• VEGA ID
  OTTHUMG00000086933

Gene info

• Identity
  HGNC:123
• Gene
  ACP2
• Long gene name
  acid phosphatase 2, lysosomal
• Synonyms
  • LAP
• Synonyms name
  • lysosomal acid phosphatase
• GenBank acession
  • X15525
• Locus
  11p11.2
• Discovery year
  1986-01-01
• Entrez gene record
  53
• Pubmed identfication
  • 975882
• RefSeq identity
  • NM_001610
• Classification
  • Acid phosphatases
• VEGA ID
  OTTHUMG00000166949

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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