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Synonyms
Cathepsin S, CTSS
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Alternative_names
Cathepsin S, CTSS
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Description
A lysosomal cysteine protease participating in the degradation of antigenic proteins to peptides
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Recombinant
Yes
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Source
HEK 293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Purity
≥95%
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Activity Specifications test method
>1000 mU (1U = 1 µmole/min/mg) as determined by Cathepsin S Activity Assay Kit (K144-100).
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Biological activity
>1000 mU (1U = 1 µmole/min/mg) as determined by Cathepsin S Activity Assay Kit (K144-100).
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Results
>1000 mU (1U = 1 µmole/min/mg) .
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Molecular Weight
~ 37 kDa
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Storage Temp
-80°C
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Shipping
Dry Ice
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Shelf Life
6 months
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Concentration
0.2 uM
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Appearance
Liquid
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Physical form description
A 0.2 uM filtered solution of 20mM MES, 150mM NaCl, 10% Glycerol, pH 5.5.
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Background Information
Cathepsin S (CTSS) is a lysosomal cysteine protease of the papain family and may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. CTSS is synthesized as inactive precursor of 331 amino acids consisting of a 15-aa signal peptide, a propeptide of 99 aa, and a mature polypeptide of 217 aa. It is activated in the lysosomes by a proteolytic cleavage of the propeptide. The deduced amino acid sequence contains only one potential N-glycosylation site located in the propeptide. Compared with the abundant cathepsins B, L and H, cathepsin S shows a restricted tissue distribution, with highest levels in spleen, heart, and lung. In addition, evidences indicated that cathepsin S generates A beta from amyloidogenic fragments of beta APP in the endosomal/lysosomal compartment, and is implicated in the pathogenesis of Alzheimer’s disease (AD) and Down Syndrome (DS).
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Amino acid sequence
QLHKDPTLDHHWHLWKKTYGKQYKEKNEEAVRRLIWEKNLKFVMLHNLEHSMGMHSYDLGMNHLGDMTSEEVMSLMSSLRVPSQWQRNITYKSNPNWILPDSVDWREKGCVTEVKYQGSCGACWAFSAVGALEAQLKLKTGKLVSLSAQNLVDCSTEKYGNKGCNGGFMTTAFQYIIDNKGIDSDASYPYKAMDQKCQYDSKYRAATCSKYTELPYGREDVLKEAVANKGPVSVGVDARHPSFFLYRSGVYYEPSCTQNVNHGVLVVGYGDLNGKEYWLVKNSWGHNFGEEGYIRMARNKGNHCGIASFPSYPEIVDHHHHHH
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Gene
CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
