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Immunogen
A synthesized peptide derived from human RhoA
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Antibody s target
Human RhoA
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Antibody s full description
Rabbit Anti-Human RhoA Polyclonal
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Primary research fields
Cell Signaling, Cancer
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Aplysia ras related homolog 12 Antibody, ARH12 Antibody, ARHA Antibody, H12 Antibody, Ras homolog family member A Antibody, Rho A Antibody, RHO H12 Antibody, RHOA Antibody, Small GTP binding protein Rho A Antibody, Transforming protein Rho A Antibody
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Verified applications
WB, IHC
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~22kDa, endogenous levels of total RhoA
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1332 was sufficient for detection of RhoA in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Membrane, Cytoplasm, Cytoskeleton
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
RhoA is a small GTPase protein of the Rho family. The Rho family acts as molecular swithces and regulators of processes such as cell migration, proliferation and differentiation. RhoA specifically regulates actomyosin contractility, cytokinesis, focal adhesion assembly and cell polarity.
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Bibliography
1. Bi D., et al. (2005) Circ. Res. 96: 890-897. 2. Kimura K., et al. (2000) J. Biol. Chem. 275: 17233-17236. 3. Barry S.T. and Critchley D.R. (1994) J. Cell Sci. 107 ( Pt 7): 2033-2045. 4. Van Keymeulen A., et al. (2006) J. Cell Biol. 174: 437-445.
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Released date
6/Sep/2016
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NCBI number
NP_001655.1
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Gene number
387
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Protein number
P61586
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Human HeLa cell lysates showing detection of ~22kDa RhoA protein using Rabbit Anti-RhoA Polyclonal Antibody (SPC-1332). Lane 1: Human HeLa treated with the immunizing peptide. Lane 2: Human HeLa. Primary Antibody: Rabbit Anti-RhoA Polyclonal Antibody (SPC-1332) at 1:1000. Predicted/Observed Size: ~22kDa. Rabbit Anti-RhoA Antibody used in Western blot (WB) on HeLa cell lysates (SPC-1332)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
RHOA-IT1, RHOA, MRI1
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Short name
Rabbit Anti- RhoA Polyclonal:
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
Biotin
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman ras homolog family member A polyclonal: biotinilated
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
ras homolog family member A, ARH12 and ARHA and RHO12 and RHOH12, RHOA and IDBG-34732 and ENSG00000067560 and 387, myosin binding, Cell surfaces, 4930544G11Rik and IDBG-153447 and ENSMUSG00000036463 and 67653, RHOA and IDBG-645998 and ENSBTAG00000004279 and 338049
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