Refer to PubMed
Net weight (g)
Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Human, Mouse, Rat
Representative figure legend
Western blot analysis of Human HeLa cell lysates showing detection of ~22kDa RhoA protein using Rabbit Anti-RhoA Polyclonal Antibody (SPC-1332). Lane 1: Human HeLa treated with the immunizing peptide. Lane 2: Human HeLa. Primary Antibody: Rabbit Anti-RhoA Polyclonal Antibody (SPC-1332) at 1:1000. Predicted/Observed Size: ~22kDa. Rabbit Anti-RhoA Antibody used in Western blot (WB) on HeLa cell lysates (SPC-1332)
A synthesized peptide derived from human RhoA
Antibody's other name
Aplysia ras related homolog 12 Antibody, ARH12 Antibody, ARHA Antibody, H12 Antibody, Ras homolog family member A Antibody, Rho A Antibody, RHO H12 Antibody, RHOA Antibody, Small GTP binding protein Rho A Antibody, Transforming protein Rho A Antibody
Antibody's recommended dilutions for use
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
To be tested
Primary research fields
Cell Signaling, Cancer
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Total weight (kg)
RhoA is a small GTPase protein of the Rho family. The Rho family acts as molecular swithces and regulators of processes such as cell migration, proliferation and differentiation. RhoA specifically regulates actomyosin contractility, cytokinesis, focal adhesion assembly and cell polarity.
Certificate of analysis
A 1:1000 dilution of SPC-1332 was sufficient for detection of RhoA in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody's full description
Rabbit Anti-Human RhoA Polyclonal
Country of production
Antibody's purified from
Peptide Affinity Purified
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To be tested
Antibody in cell
Cell Membrane, Cytoplasm, Cytoskeleton
1. Bi D., et al. (2005) Circ. Res. 96: 890-897. 2. Kimura K., et al. (2000) J. Biol. Chem. 275: 17233-17236. 3. Barry S.T. and Critchley D.R. (1994) J. Cell Sci. 107 ( Pt 7): 2033-2045. 4. Van Keymeulen A., et al. (2006) J. Cell Biol. 174: 437-445.
Detects ~22kDa, endogenous levels of total RhoA
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