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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide which is a part of part of the glutamine synthetase/guanido kinase superfamily catalytic region chosen from various available sequences, including Arabidopsis thaliana GLN2, UniProt: Q43127, TAIR: AT5G35630
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 400 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS08 295 GLN1 GLN2 | GS1 glutamine synthetase global antibody
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Recommended dilutions for use
1:5000 on 0.5-5 ug protein/lane with ECL Advance detection (WB)
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Molecular weight expected аpparent
47 | 44-45 kDa
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Verified reactivity
Arabidopsis thaliana, Oryza sativa, Pisum sativum, Spinacia oleracea
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Possible reactivity
dicots including: Brassica napus, Glycine max, Phaseolus vulgaris, monoctos including: Hordeum vulgare, Triticum, aestivum,Zea mays,trees: Pinus sylvestris, Populus sp., moss: Physcomitrella patens,
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No reactivity
diatoms
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Supplementary information
to be added when available
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References
Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.Lee et al. (2013). Stromal protein degradation is incomplete in Arabidopsis thaliana autophagy mutants undergoing natural senescence. BMC Res Notes, Jan 17. Hu and Li (2012). The amino-terminal domain of chloroplast Hsp93 is important for its membrane association and functions in vivo. Plant Physiol. Apr;158(4):1656-65. doi: 10.1104/pp.112.193300. Epub 2012 Feb 21.
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Scientific context
Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN2 is encoded by a single gene and is highly abundant in mesophyll cells of leaves for the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts. GLN2 is a target for thioredoxin.
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Notes
None
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Protein number
Q43127
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TAIR number
AT5G35630
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