HAMSTER ANTI MOUSE CD49e:Biotin Antibody

• Catalog number
  GEN210444
• Price
  Please ask
• Size
  100ug

• Also known as
  CD49e
• Other names
  integrin alpha-5 preproprotein; Integrin alpha-5; integrin alpha-5; integrin alpha-F; CD49 antigen-like family member E; fibronectin receptor subunit alpha; fibronectin receptor alpha polypeptide; integrin alpha 5 (fibronectin receptor alpha); CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  CD49e
• Gene name synonims
  N/A
• Other gene names
  Itga5; Itga5; Fnra; VLA5; Cd49e; N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  IgG
• Clone
  HM alpha 5
• Host organism
  Hamster
• Species reactivity
  Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  CD49e This item recognises the mouse integrin alpha 5 subunit (CD49e). CD49e is a type I membrane protein that associates non-covalently with integrin beta 1 (CD29) to form the heterodimer CD49e/CD29 (VLA-5). VLA-5 is expressed on mast cells, splenic B cells, thymocytes, T-cells and monocytes. The VLA-5 heterodimer forms the fibronectin receptor, which plays an important role in cell adhesion and migration.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  N/A
• Form Appearance
  Biotin (Purified IgG conjugated to Biotin - liquid)
• Concentration
  IgG concentration 0.1 miligram per 1 mililiter
• Storage and shipping
  Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
• Tested applications
  Flow Cytometry (FACS)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
• Properties
  If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
• Conjugation
  Biotinylated
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• French translation
  anticorps

• Gene target
  CD49e  
• Gene symbol
  ITGA5
• Short name
  ANTI MOUSE CD49e: Antibody
• Technique
  anti mouse, Antibody, Mouse, anti, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, mouses
• Host
  Hamster
• Label
  Biotin
• Species
  Mouse, Hamsters, Mouses
• Alternative name
  Anti-HAMSTER ANTI MOUSE CD49e:Biotin
• Alternative technique
  antibodies, murine

Gene info

• Identity
  HGNC:6141
• Gene
  ITGA5
• Long gene name
  integrin subunit alpha 5
• Synonyms gene
  • FNRA
• Synonyms gene name
  • integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Synonyms
  • CD49e
• Synonyms name
  • fibronectin receptor, alpha polypeptide
• Locus
  12q13.13
• Discovery year
  1988-07-19
• Entrez gene record
  3678
• Pubmed identfication
  • 2454952
• Classification
  • Integrin alpha subunits
  • CD molecules
• VEGA ID
  OTTHUMG00000169841

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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