TCR beta monoclonal antibody

  • Catalog number
    12-571-C100
  • Price
    Please ask
  • Size
    100ug
  • Long name
    Hamster Monoclonal Antibody against Mouse TCR beta, Clone: H57-597
  • Antigen
    TCR beta (mouse)
  • Species reactivity
    Mouse (Mus musculus)
  • Negative species reactivity
    at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
  • Clonality
    Monoclonal Antibody (Mab)
  • Clone
    H57-597
  • Applications
    FC, IP, IHC(P), IHC(F), ICC, FUNC
  • Immunogen
    Affinity purified TCR from DO-11.10 T cell hybridoma_x000D_
  • Format conjugation
    low endotoxin, unconjugated
  • Specificity
    The Armenian hamster monoclonal antibody H57-597 reacts specifically with beta subunit of TCR alpha/beta. This antibody does not crossreact with TCR gamma/delta type and has been used as TCR alpha/beta phenotypic marker._x000D_
  • Research area
    CD and Related Antigens & MHC (Rodent)
  • Concentration
    1 mg/ml
  • Storage buffer
    Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
  • Shipping and storage
    Hamster Monoclonal Antibody against Mouse TCR beta, Clone: H57-597 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
  • Background
    The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression._x000D_
  • Purity
    > 95% (by SDS-PAGE)
  • Purification method
    Purified by protein-A affinity chromatography
  • References
    _x000D_ , *Oliver PM, Cao X, Worthen GS, Shi P, Briones N, MacLeod M, White J, Kirby P, Kappler J, Marrack P, Yang B: Ndfip1 protein promotes the function of itch ubiquitin ligase to prevent T cell activation and T helper 2 cell-mediated inflammation. Immunity. 2006 Dec;25(6):929-40._x000D_ , *Rozdzial MM, Kubo RT, Turner SL, Finkel TH: Developmental regulation of the TCR zeta-chain. Differential expression and tyrosine phosphorylation of the TCR zeta-chain in resting immature and mature T lymphocytes. J Immunol. 1994 Aug 15;153(4):1563-80._x000D_ , *Kishimoto H, Kubo RT, Yorifuji H, Nakayama T, Asano Y, Tada T: Physical dissociation of the TCR-CD3 complex accompanies receptor ligation. J Exp Med. 1995 Dec 1;182(6):1997-2006. _x000D_ , *van der Heyde HC, Elloso MM, Chang WL, Kaplan M, Manning DD, Weidanz WP: Gamma delta T cells function in cell-mediated immunity to acute blood-stage Plasmodium chabaudi adami malaria. J Immunol. 1995 Apr 15;154(8):3985-90._x000D_ , *McCormack JE, Kappler J, Marrack P: Stimulation with specific antigen can block superantigen-mediated deletion of T cells in vivo. Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2086-90._x000D_ , *Skeen MJ, Ziegler HK: Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection. J Exp Med. 1993 Sep 1;178(3):971-84._x000D_ , *Skeen MJ, Ziegler HK: Intercellular interactions and cytokine responsiveness of peritoneal alpha/beta and gamma/delta T cells from Listeria-infected mice: synergistic effects of interleukin 1 and 7 on gamma/delta T cells. J Exp Med. 1993 Sep 1;178(3):985-96._x000D_ , *Gascoigne NR: Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3. J Biol Chem. 1990 Jun 5;265(16):9296-301._x000D_ , *Goodman T, Lefrancois L: Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function. J Exp Med. 1989 Nov 1;170(5):1569-81._x000D_ , *Kubo RT, Born W, Kappler JW, Marrack P, Pigeon M: Characterization of a monoclonal antibody which detects all murine alpha beta T_x000D_ cell receptors. J Immunol. 1989 Apr 15;142(8):2736-42._x000D_ _x000D_
  • Properties
    If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • French translation
    anticorps
  • Gene target
    TCR   beta   monoclonal  
  • Short name
    Anti-TCR beta Mab
  • Technique
    Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
  • Isotype
    IgG
  • Alternative name
    Hamster Monoclonal to TCR beta (mouse)
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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