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Synonyms
EPCAM, DIAR5, DIAR-5, EGP-2, EGP2, EGP314, EGP-314, EGP40, EGP-40, ESA, GA733-2, HNPCC8, HNPCC-8, KS1/4, KSA, M4S1, MIC18, MIC-18, MK-1, MK1, TACSTD1, TACSTD-1, TROP1, TROP-1, CD326, CD-326, Epithelial cell adhesion molecule, tumor-associated calcium signal transducer 1, cluster of differentiation 326.
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Alternative_names
EPCAM, DIAR5, DIAR-5, EGP-2, EGP2, EGP314, EGP-314, EGP40, EGP-40, ESA, GA733-2, HNPCC8, HNPCC-8, KS1/4, KSA, M4S1, MIC18, MIC-18, MK-1, MK1, TACSTD1, TACSTD-1, TROP1, TROP-1, CD326, CD-326, Epithelial cell adhesion molecule, tumor-associated calcium signal transducer 1, cluster of differentiation 326.
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Description
A pan-epithelial differentiation antigen that is expressed on almost all carcinomas
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human Cathepsin V at 10 ug/mL (100 ul/well) can bind biotinylated EpCAM. The EC50 of biotinylated EpCAM is 50-70 ng/mL.
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Results
Measured by its binding ability in a functional ELISA. Immobilized recombinant human Cathepsin V at 10 ug/mL (100 ul/well) can bind biotinylated EpCAM. The EC50 of biotinylated EpCAM is 50-70 ng/mL.
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Molecular Weight
This protein contains a poly histidine tag at C-terminus and has a calculated MW of 29 kDa after removal of signal peptide. The predicted N-terminus is Gln24. In DTT-reduced SDS-PAGE, rh EpCAM protein migrates as 33-36 kDa poly peptide due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background Information
EpCAM is also known as CO171A,; EGP; EGP40; GA7332; KSA; M4S; MIC18; MK1; TROP1; hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy. Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
