Human CellExp™ CD166/ ALCAM, human recombinant
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Catalog number7437-10
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PricePlease ask
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Size10 ug
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SynonymsCD166, ALCAM, CD6L, KG-CAM, MEMD
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Alternative_namesCD166, ALCAM, CD6L, KG-CAM, MEMD
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DescriptionA cell adhesion molecule that binds to CD6
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RecombinantYes
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SourceHEK293 cells
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Biological activityMeasured by its binding ability in a functional ELISA. Immobilized rh CD166 / ALCAM at 10 ug/ml (100 ul/well) can bind mouse CD6 Fc Chimera. The EC50 of mouse CD6 Fc Chimera is 0.1-0.22 ug/ml.
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ResultsMeasured by its binding ability in a functional ELISA. Immobilized rh CD166 / ALCAM at 10 ug/ml (100 ul/well) can bind mouse CD6 Fc Chimera. The EC50 of mouse CD6 Fc Chimera is 0.1-0.22 ug/ml.
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Molecular WeightThis protein is fused with 6×his tag at the C-terminus, and has a calculated MW of 56.8 kDa. The predicted N-terminus is Trp 28. DTT-reduced Protein migrates as 65-80 kDa in SDS-PAGE due to glycosylation.
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from 0.22 um filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background InformationCD166 antigen is a 100-105 kDa type I transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins, which is also known as activated leukocyte cell adhesion molecule (ALCAM) in human, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. CD166 is a cell adhesion molecule that binds to CD6. CD166 involved in neurite extension by neurons via heterophilic and homophilic interactions.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolALCAM
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Short nameCellExp™ CD166/ ALCAM, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHumans
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Alternative nameH. sapiens CellExp™ CD166/ activated leukocyte cellular adhesion molecule, H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetactivated leukocyte cell adhesion molecule, CD166 and MEMD, ALCAM and IDBG-48383 and ENSG00000170017 and 214, protein binding, Cell surfaces, Alcam and IDBG-165765 and ENSMUSG00000022636 and 11658, ALCAM and IDBG-631637 and ENSBTAG00000000088 and 281614
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Gene info
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Identity
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Gene
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Long gene nameactivated leukocyte cell adhesion molecule
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Synonyms gene name
- activated leucocyte cell adhesion molecule
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-01-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ig-like cell adhesion molecule family
- C2-set domain containing
- V-set domain containing
- CD molecules
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data