Mouse Anti-Mouse Protocadherin Gamma A (pan) Monoclonal IgG2b, Clone S144-32

• Catalog number
  SMC-453D-A655
• Price
  Please ask
• Size
  100 µg

• Clone
  S144-32
• Immunogen
  Fusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma-protocadherin-A3. Human: 80% identity (68/84 amino acids identical) >75% identity with other Gamma-protocadherin-A proteins
• Antibody s full description
  Mouse Anti-Mouse Protocadherin Gamma A Monoclonal IgG2b Antibody, Clone: S144-32: ATTO 655
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  PCDH gamma A Antibody, PCDH-gamma-A Antibody, PCDHGA Antibody, Protocadherin gamma A Antibody, Protocadherin gamma subfamily A Antibody, Gamma Protocadherin A (pan) Antibody, Pan-Gamma-Protocadherin-A Antibody, Pan Gamma Protocadherin A Antibody
• Raised in
  Mouse
• Antibody s target
  Protocadherin Gamma A
• Primary research fields
  Neuroscience, Cell Structure, Membrane Markers
• Brandname
  None
• Antibodies applications
  WB, ICC/IF
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH 7.4, 50% glycerol, 0.1% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~100kDa. Cross-reacts with other Gamma protocadherin-A proteins. No cross-reactivity against Gamma protocadherin-B or -C proteins.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  1 µg/ml of SMC-453 was sufficient for detection of Protocadherin Gamma A (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Cell Membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
• Released date
  29-Oct-2013
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_291064.1
• Gene number
  93711
• Protein number
  Q91XY5
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody, Clone S144-32 (SMC-453). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody (SMC-453) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Protocadherin Gamma A (pan) Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 showing detection of ~100 kDa Protocadherin Gamma A (pan) protein using Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody, Clone S144-32 (SMC-453). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody (SMC-453) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa. Mouse Anti-Protocadherin Gamma A (pan) Antibody [S144-32] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-453) Mouse Anti-Protocadherin Gamma A (pan) Antibody [S144-32] used in Western Blot (WB) on Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 (SMC-453)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Protocadherin   Gamma   pan   Monoclonal   IgG2b   S144-32  
• Gene symbol
  IGKV1D-32, SNORD115-32, PPP1R1B, IGLV3-32, IGHV3-32, IGKV1-32, CUTC, ZNF138, LINC01234, LUARIS
• Short name
  Mouse Anti-Mouse Protocadherin Gamma A (pan) Monoclonal IgG2b, Clone S144-32
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG2b, IgG2b
• Label
  ATTO 655
• Species
  Mouse, Mouses
• Alternative name
  Mouse Antibody toMouse Protocadherin g A (pan) monoclonal IgG2b, clonality S144-32
• Alternative technique
  murine, antibodies
• Clone name
  S144-32

Gene info

• Identity
  HGNC:5752
• Gene
  IGKV1D-32
• Long gene name
  immunoglobulin kappa variable 1D-32 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 1D-32
• GenBank acession
  • X71896
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28897
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151614

Gene info

• Identity
  HGNC:33051
• Gene
  SNORD115-32
• Long gene name
  small nucleolar RNA, C/D box 115-32
• Synonyms
  • HBII-52-32
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033806
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003347.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:9287
• Gene
  PPP1R1B
• Long gene name
  protein phosphatase 1 regulatory inhibitor subunit 1B
• Synonyms gene name
  • protein phosphatase 1, regulatory (inhibitor) subunit 1B
• Synonyms
  • DARPP-32
  • FLJ20940
• Synonyms name
  • dopamine and cAMP regulated phosphoprotein
• GenBank acession
  • AI124650
• Locus
  17q12
• Discovery year
  1993-01-22
• Entrez gene record
  84152
• Pubmed identfication
  • 8120638
• RefSeq identity
  • NM_032192
• Classification
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000133210

Gene info

• Identity
  HGNC:5914
• Gene
  IGLV3-32
• Long gene name
  immunoglobulin lambda variable 3-32 (non-functional)
• Synonyms gene name
  • immunoglobulin lambda variable 3-32
• GenBank acession
  • Z73645
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28787
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151166

Gene info

• Identity
  HGNC:5595
• Gene
  IGHV3-32
• Long gene name
  immunoglobulin heavy variable 3-32 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 3-32
  • immunoglobulin heavy variable 3-32 pseudogene
• GenBank acession
  • M99664
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28435
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152063

Gene info

• Identity
  HGNC:5736
• Gene
  IGKV1-32
• Long gene name
  immunoglobulin kappa variable 1-32 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 1-32
  • immunoglobulin kappa variable 1-32 pseudogene
• GenBank acession
  • X71883
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28934
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151687

Gene info

• Identity
  HGNC:24271
• Gene
  CUTC
• Long gene name
  cutC copper transporter
• Synonyms gene name
  • cutC copper transporter homolog (E. coli)
• Synonyms
  • CGI-32
• GenBank acession
  • AF132966
• Locus
  10q24.2
• Discovery year
  2004-04-20
• Entrez gene record
  51076
• Pubmed identfication
  • 16182249
• RefSeq identity
  • NM_015960
• VEGA ID
  OTTHUMG00000018889

Gene info

• Identity
  HGNC:12922
• Gene
  ZNF138
• Long gene name
  zinc finger protein 138
• Synonyms gene name
  • zinc finger protein 138 (clone pHZ-32)
• Synonyms
  • pHZ-32
• GenBank acession
  • U09847
• Locus
  7q11.21
• Discovery year
  1993-02-11
• Entrez gene record
  7697
• RefSeq identity
  • NM_006524
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000156603

Gene info

• Identity
  HGNC:49757
• Gene
  LINC01234
• Long gene name
  long intergenic non-protein coding RNA 1234
• Synonyms
  • onco-lncRNA-32
• Locus
  12q24.13
• Discovery year
  2014-02-14
• Entrez gene record
  100506465
• Pubmed identfication
  • 24522499
  • 28484081
  • 28978132
• RefSeq identity
  • NR_110026
• Classification
  • Long intergenic non-protein coding RNAs
• VEGA ID
  OTTHUMG00000169644

Gene info

• Identity
  HGNC:40992
• Gene
  LUARIS
• Long gene name
  lncRNA upregulator of antiviral response interferon signaling
• Synonyms
  • lncRNA#32
  • SCAT6
• Synonyms name
  • S-phase cancer-associated transcript 6
• Locus
  7p13
• Discovery year
  2017-03-29
• Entrez gene record
  100506895
• Pubmed identfication
  • 27582466
  • 29491376
• RefSeq identity
  • NR_038276
• Classification
  • Long non-coding RNAs with non-systematic symbols

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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