This antibody has been succesfully tested for use in following methods: Western blot
Target of this antibody: UCHL1
Formulation of this antibody: 50 µg of antibody in PBS containing 10% glycerol
Alternative names of antibody target: Neuron cytoplasmic protein 9.5, PARK5, PGP9.5, PGP 9.5, Ubiquitin carboxyl-terminal, hydrolase isozyme L1, Ubiquitin thioesterase L1, UCH-L1, Ubiquitin thioesterase L1.
Recommended usage: Western blot: Robust detection of 100 ng of recombinant protein was possible when antibody was used at a final concentration of 5 μg/mL
Storage information: Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions: gel pack
Antibody host: Chicken
This antibody reacts with: Human
Clonality of antibody: Polyclonal
Isotype: Chicken IgG
Purification method: Purified from egg yolk
Immunogene sequence: Recombinant full length protein
How to handle this antibody: The antibody solution should be gently mixed before use.
Background information: Protein ubiquitination and Deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs). DUBs are categorized into 5 subfamilies: USP, UCH, OTU, MJD, and JAMM. UCHL1, UCHL3, UCHL5/UCH37, and BRCA-1-associated protein-1 (BAP1) belong to the UCH family of DUBs, which all possess a conserved catalytic domain (UCH domain) of about 230 amino acids. Although UCHL1 and UCHL3 are the most closely related UCH family members with about 53% identity, their biochemical properties differ in that UCHL1 binds monoubiquitin and UCHL3 shows dual specificity toward both ubiquitin (Ub) and NEDD8, a Ub-like molecule. UCHL1 (PGP9.5) is a 25 kDa protein; it is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It comprises>1% of total brain protein but is almost absent from other tissues. It has been implicated both in Parkinson’s disease and in lung cancer.
Form of antibody: Liquid
Concentration of antibody: N/A
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation anticorps