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Human Endothelin ELISA Kit 96 well plate

Human Endothelin ELISA Kit 96 well plate is available 27 times from Bioma labs

EKU09462 | Human Endothelin 1 (EDN1) CLIA Kit, 96 well plate size: 96T | 1,221.70 USD

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EKU09462 | Human Endothelin 1 (EDN1) CLIA Kit, 96 well plate size: 5x96T | 5,171.33 USD

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EKU09462 | Human Endothelin 1 (EDN1) CLIA Kit, 96 well plate size: 10x96T | 9,351.01 USD

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EKU03887 | Human Endothelin 1 (EDN1) ELISA Kit, 96 well plate size: 5x96T | 3,937.52 USD

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EKU03887 | Human Endothelin 1 (EDN1) ELISA Kit, 96 well plate size: 96T | 932.32 USD

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EKU03887 | Human Endothelin 1 (EDN1) ELISA Kit, 96 well plate size: 10x96T | 7,115.87 USD

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EKU08407 | Human Endothelin 2 (EDN2) ELISA Kit, 96 well plate size: 96T | 1,027.97 USD

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EKU08407 | Human Endothelin 2 (EDN2) ELISA Kit, 96 well plate size: 5x96T | 4,318.92 USD

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EKU08407 | Human Endothelin 2 (EDN2) ELISA Kit, 96 well plate size: 10x96T | 7,803.61 USD

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EKU03892 | Human Endothelin Converting Enzyme 1 (ECE1) ELISA Kit, 96 well plate size: 5x96T | 3,937.52 USD

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EKU03892 | Human Endothelin Converting Enzyme 1 (ECE1) ELISA Kit, 96 well plate size: 10x96T | 7,115.87 USD

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EKU03892 | Human Endothelin Converting Enzyme 1 (ECE1) ELISA Kit, 96 well plate size: 96T | 932.32 USD

Catalog number EKU03892
Supplier bioma
Price 932.32 USD
Size 96T
Verified reactivity Homo sapiens (Human)
Detection limits 31.25-2000 pg/mL
Sensitivity limit 11,8 pg/mL
Samples to be analyzed Serum, plasma and other biological fluids
Assay duration 3 hours
Verified applications ELISA
Assay class Sandwich
ELISA detection Colorimetric
Protein number P42892
Gene number 1889
Gene name Endothelin Converting Enzyme 1
Alternate gene name Please refer to NCBI
Use for 8 months
ELISA's specificity This assay has high sensitivity and excellent specificity for detection of Endothelin Converting Enzyme 1 (ECE1). No significant cross-reactivity or interference between Endothelin Converting Enzyme 1 (ECE1) and analogues was observed.
Assay principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelin Converting Enzyme 1 (ECE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endothelin Converting Enzyme 1 (ECE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endothelin Converting Enzyme 1 (ECE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endothelin Converting Enzyme 1 (ECE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Precision of the test Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelin Converting Enzyme 1 (ECE1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelin Converting Enzyme 1 (ECE1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
ELISA's stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
QC The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research main area Enzyme & Kinase;Cardiovascular biology;
Warnings The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Gene targetEndothelin Converting ECE1
Short name Endothelin Converting Enzyme 1 (ECE1) ELISA Kit, 96
Technique ELISA kit, Enzyme, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Alternative name H. sapiens Endothelin Converting Enzyme 1 (endothelin converting enzyme 1) Enzyme-linked immunosorbent assay reagent, 96 well plate
Alternative technique enzymes
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832, endothelin converting enzyme 1, ECE, ECE1 and IDBG-93094 and ENSG00000117298 and 1889, metal ion binding, Cell surfaces, Ece1 and IDBG-199213 and ENSMUSG00000057530 and 230857, ECE1 and IDBG-645619 and ENSBTAG00000002977 and 281133
1. Gene info
Identity 3146
Gene ECE1
Long gene name endothelin converting enzyme 1
Synonyms gene
  • ECE
GenBank acession
  • D49471
Locus 1p36.12
Discovery year 1995-12-08
Entrez gene record 1889
Pubmed identfication
  • 7805846
  • 7864876
  • 17592116
RefSeq identity
  • NM_001397
Classification
  • M13 metallopeptidases
Havana BLAST/BLAT OTTHUMG00000002625
Product images
    #Human#.jpg

EKU08428 | Human Endothelin Converting Enzyme 2 (ECE2) ELISA Kit, 96 well plate size: 96T | 1,027.97 USD

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EKU08428 | Human Endothelin Converting Enzyme 2 (ECE2) ELISA Kit, 96 well plate size: 5x96T | 4,318.92 USD

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EKU08428 | Human Endothelin Converting Enzyme 2 (ECE2) ELISA Kit, 96 well plate size: 10x96T | 7,803.61 USD

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EKU03893 | Human Endothelin Receptor A (ETRA) ELISA Kit, 96 well plate size: 96T | 979.54 USD

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EKU03893 | Human Endothelin Receptor A (ETRA) ELISA Kit, 96 well plate size: 5x96T | 4,153.04 USD

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EKU03893 | Human Endothelin Receptor A (ETRA) ELISA Kit, 96 well plate size: 10x96T | 7,509.38 USD

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EKU03894 | Human Endothelin Receptor B (ETRB) ELISA Kit, 96 well plate size: 10x96T | 7,509.38 USD

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EKU03894 | Human Endothelin Receptor B (ETRB) ELISA Kit, 96 well plate size: 5x96T | 4,153.04 USD

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EKU03894 | Human Endothelin Receptor B (ETRB) ELISA Kit, 96 well plate size: 96T | 979.54 USD

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EKC33571 | Human Endothelin-1 receptor (EDNRA) ELISA Kit, 96 well plate size: 96T | 989.22 USD

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EKC33571 | Human Endothelin-1 receptor (EDNRA) ELISA Kit, 96 well plate size: 10x96T | 7,023.85 USD

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EKC33571 | Human Endothelin-1 receptor (EDNRA) ELISA Kit, 96 well plate size: 5x96T | 3,774.06 USD

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EKC33573 | Human Endothelin-3 (EDN3) ELISA Kit, 96 well plate size: 5x96T | 3,774.06 USD

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EKC33573 | Human Endothelin-3 (EDN3) ELISA Kit, 96 well plate size: 96T | 989.22 USD

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EKC33573 | Human Endothelin-3 (EDN3) ELISA Kit, 96 well plate size: 10x96T | 7,023.85 USD

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Alternate protein number Please refer to SwissProt
Estimated production time 7-11 business days
Shipping requirements Blue ice
Storage recommendation -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
ELISA's cross-reactivity This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Protocol Please see ELISA's datasheet, otherwise contact us
Notes For research use only. Not for diagnostic procedures.
Species Human
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Endothelin Converting Enzyme 1 (ECE1) ELISA Kit, 96 | Techni
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  • EDN1
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