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Rabbit Anti Human GABARAP Polyclonal Streptavidin

Rabbit Anti Human GABARAP Polyclonal Streptavidin is available 2 times from Stressmark antibodies labs

SPC-620D-STR | Rabbit Anti-Human GABARAP Polyclonal: Streptavidin size: 100 µg | 445.13 USD

Immunogen Synthetic peptide from the N-terminal of human GABARAP
Antibody's specificity Detects ~14-16kDa.
Certificate of analysis A 1:1000 dilution of SPC-620 was sufficient for detection of GABARAP on mouse kidney lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Representative figure linkmore | details | see here
Representative figure legend Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Endomembrane System, Cytoplasm, Golgi Apparatus Membrane, Cytoplasmic Vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAP Antibody (D) Composite. | Western blot analysis of Human 293T showing detection of ~13.9kDa GABARAP protein using Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620). Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~13.9kDa. Other Band(s): ~40kDa (Complex). | Western blot analysis of Mouse Kidney showing detection of ~13.9kDa GABARAP protein using Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620). Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody (SPC-620) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~13.9kDa. Other Band(s): ~40kDa (Complex). Rabbit Anti-GABARAP Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Neuroblastoma cell line (SK-N-BE) (SPC-620) | Rabbit Anti-GABARAP Antibody used in Western blot (WB) on 293T (SPC-620) | Rabbit Anti-GABARAP Antibody used in Western blot (WB) on Kidney (SPC-620)
1. Gene info
Identity4067
Synonyms gene name
  • GABA(A) receptor-associated protein
Synonyms
GenBank acession
Locus17p13.1
Discovery year1999-09-29
Pubmed identfication
Havana BLAST/BLATOTTHUMG00000102156
MeSH Data
Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
Product images
    #Human#.jpg

SPC-621D-STR | Rabbit Anti-Human GABARAP Polyclonal: Streptavidin size: 100 µg | 445.13 USD

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Antibody's target Human GABARAP
Antibody's full description Rabbit Anti-Human GABARAP Polyclonal
Primary research fields Neuroscience, Neurotransmitter Receptors, GABA Receptors
Antibody's category Polyclonal Antibodies
Antibody's other name ATG8A Antibody, GABARAP A Antibody, GABA(A) Receptor Associated Protein Antibody, MM46 Antibody
Verified applications WB, ICC/IF
Raised in Rabbit
Antibody's reactivity Human, Mouse
Antibody's recommended dilutions for use WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody's purified from Peptide Affinity Purified
Recommended buffer for storage PBS, 50% glycerol, 0.09% sodium azide
Antibody's concentration 1 mg/ml
Storage recommendations -20°C
Shipping recommendations Blue Ice or 4°C
Antibody in cell Endomembrane System, Cytoplasm, Cytoskeleton, Golgi Apparatus, Golgi Apparatus Membrane, Cytoplasmic Vesicle
Tissue specificity See included datasheet or contact our support service.
Scientific context Gamma-aminobutyric acid receptor-associated protein, or GABARAP, are lighand gated chloride channels that mediate inhibitory neurotransmission. It clusters neurotransmitter receptors by mediating its interaction with the cytoskeleton (1).
Bibliography 1. "Entrez Gene: GABARAP GABA(A) receptor-associated protein".
Released date 1/Oct/2015
NCBI number NP_009209.1
Gene number 11337
Protein number O95166
PubMed number Refer to PubMed
Tested applications To be tested
Tested reactivity To be tested
Antibody's datasheet Contact our support service to receive datasheet or other technical documentation.
Warning information Non-hazardous
Country of production Canada
Total weight (kg) 1.4
Net weight (g) 0.1
Stock availabilit In Stock
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test StressMark antibodies supplies antibodies that are for research of human proteins.
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name Oryctolagus cuniculus
Gene targetGABARAP Streptavidin
Short name Rabbit Anti- GABARAP Polyclonal: Streptavidin
Technique anti-Human, Polyclonal, Rabbit, anti
Host Rabbit
Label streptavidin
Species Human
Alternative name production species: rabbit antibody to-H. sapiens GABARAP polyclonal: biotinilated-binding streptavidin
Alternative technique polyclonals
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