H3K9acS10p Antibody
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Catalog number6809-50
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PricePlease ask
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Size50 μl
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Antibody TargetH3K9acS10p
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Target Alternative NameHistone H3
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Antibody TypePolyclonal
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Species ReactivitiesHuman.
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Immunogen SequenceKLH-conjugated synthetic peptide of Histone H3 containing acetylated lysine 9 and the phosphorylated serine 10.
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AccessionP68431
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Gene Id8350
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AppearanceColorless liquid
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FormLiquid
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FormulationIn PBS with 0.05% (W/V) sodium azide.
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PurificationWhole antiserum from rabbit
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ApplicationWestern blot, IF, ELISA, ChIP, Dot blot.
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Positive ControlIF: HeLa cells, WB: HeLa cells, ELISA: Antigen, ChIP: Human osteosarcoma cells, Dot blot: Histone peptides
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Application And UsagesIF: 1:500, WB: 1:250, ELISA: 1:1000 – 1:4000, Dot Blot: 1:20000, ChIP: 15 µl/ChIP.
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DescriptionHistones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of K9 and phosphorylation of S10 of histone H3 are associated with active gene transcription.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedVerified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedVerified
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ChIP VerifiedVerified
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Dot Blot VerifiedVerified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Short nameH3K9acS10p Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameH3K9acS10p (antibody to-)
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data