Rabbit Polyclonal IgG Anti-Beta-Defensin-1 (HBD-1), affinity purified
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Catalog numberDEF01-A
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PricePlease ask
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Size100 µg
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AvailabilityPlease contact us to check the availability
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ImmunogenSynthetic peptide, 17 AA near N-terminus of the mature HBD-1, coupled to KLH for immunisation of rabbits
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Specificityß-defensin-1 (HBD-1) whole molecule and 17AA N-terminal fraction of HBD-1, no cross-reactivity to alpha-defensin and other beta-defensins
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Species reactivityhuman, monkey, no cross-reactivity with mouse and rat
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Product presentationPeptide Sepharose purified IgG in PBS, BSA, lyophilized without preservative. Reconstitute with 0.1 ml sterile water to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.
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Product categoryPrimary Antibody
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Antibody s raised inRabbit
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Antibody s isotypeIgG
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Antibody s clonepolyclonal
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Product is supplied aslyophilisate
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Recommended concentration for use1-10 µg/ml Western Blot, 0.5-1 µg/ml ELISA, IHC 2-10 µg/ml (not tested)
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Protein concentrationN/A
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Positive controlRenal epithelia, bladder epithelia
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Tested applicationsIHC(C, P), ELISA, RIA
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Recommended secondary reagentWe recommend the VECTASTAIN Elite ABC Kit Rabbit IgG (code no. PK-6101) or BioPrime Universal Staining Kits (code no. AE005 and DA005).
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Storage methodStore at -20'°C'
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UniProt numberGo to UniProt/SwissProt
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Litterature1. Bensch K.W., Raida M., Mägert H.J., Schulz-Knappe P., and W.G. Forssmann. HBD-1: a novel beta -defensin from human plasma. FEBS Lett 1995, 368; 331-335. (original publication HBD-1) 2. Valore EV, Park CH, Quayle AJ, Wiles KR, McCray PB, Ganz T. Human beta-defensin-1: an antimicrobial peptide of urogenital tissues. J Clin Invest. 1998 Apr 15;101(8):1633-42. 3. O'Neil DA, Porter EM, Elewaut D, Anderson GM, Eckmann L, Ganz T, Kagnoff MF. Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. J Immunol. 1999 Dec 15;163(12):6718-24. 4. Ganz T. Defensins and host defense. Science. 1999 Oct 15;286(5439):420-1. 5. Shi J, Zhang G, Wu H, Ross C, Blecha F, Ganz T. Porcine epithelial beta-defensin 1 is expressed in the dorsal tongue at antimicrobial concentrations. Infect Immun. 1999 Jun;67(6):3121-7.
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TipsGentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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TestA high affinity purification column was use to purify Research by bio logo by chromatographic size exclusion.
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Description1 of protein A or G purified by bio logo will give more specificity than crude serum. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. human, mouse or other beta β- and alpha α- defensins are expressed by neutrophils ( α- ), lymphocytes and epithelial cells. DEFA DEFB genes defend the host against pathogens. GENTAUR supplies anti-defensins, monclonals, polyclonals antisera and recombinant DEFs or peptides of defensin.
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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GroupPolyclonals and antibodies
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolDEFB1, HBD, DEFB4A, DEFB103A, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1
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Short nameResearch
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TechniquePolyclonal, affinity, purified, Rabbit, anti-, anti, IgG, antibody to, antibodies, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. purifications
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HostRabbit, Rabbits
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IsotypeIgG, IgG
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Alternative nameBeta-Defensin-1 (HBD-1), affinity purified
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Alternative techniquepolyclonals, pure, rabbit-anti, antibodies, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene namedefensin beta 1
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Synonyms gene name
- defensin, beta 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-05-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Defensins, beta
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namehemoglobin subunit delta
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Synonyms gene name
- hemoglobin, delta
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Hemoglobin subunits
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namedefensin beta 4A
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Synonyms gene
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Synonyms gene name
- defensin, beta 2
- defensin, beta 4
- defensin, beta 4A
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-08-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
- Defensins, beta
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namedefensin beta 103A
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Synonyms gene
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Synonyms gene name
- defensin, beta 3
- defensin, beta 103A
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Defensins, beta
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 7-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 101-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR101 family
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data