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Available ordering format
Liquid
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Immunogen
KDEL containing peptide
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at +4°C
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Verified applications
western blot (WB), immunohistochemistry, paraffin-embedded (IHC)
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Connected products
AS13 2665 | anti-KDEL | endoplasmic reticulum retention signal (50 µg), rabbit antibodyAS15 3011 | anti-KDEL | endoplasmic reticulum retention signal, ALP conjugated, rabbit antibodyAS15 3013 | anti-KDEL | endoplasmic reticulum retention signal, APC conjugated, rabbit antibody AS15 3014 | anti-KDEL | endoplasmic reticulum retention signal, Biotin conjugated, rabbit antibodyAS15 3016 | anti-KDEL | endoplasmic reticulum retention signal, HRP conjugated, rabbit antibody AS15 3017 | anti-KDEL | endoplasmic reticulum retention signal, Streptavidin conjugated, rabbit antibodyAS15 3003 | anti-KDEL | endoplasmic reticulum retention signal, ATTO 390 conjugated, rabbit antibodyAS15 3004 | anti-KDEL | endoplasmic reticulum retention signal, ATTO 488 conjugated, rabbit antibodyAS15 3005 | anti-KDEL | endoplasmic reticulum retention signal, ATTO 565 conjugated, rabbit antibodyAS15 3006 | anti-KDEL | endoplasmic reticulum retention signall, ATTO 594 conjugated, rabbit antibodyAS15 3007 | anti-KDEL | endoplasmic reticulum retention signal, ATTO 633 conjugated, rabbit antibodyAS15 3008 | anti- KDEL | endoplasmic reticulum retention signal, ATTO 655 conjugated, rabbit antibodyAS15 3009 | anti-KDEL | endoplasmic reticulum retention signal, ATTO 680 conjugated, rabbit antibodyAS15 3010 | anti-KDEL | endoplasmic reticulum retention signal, ATTO 700 conjugated, rabbit antibodyAS10 683-25 | anti-HDEL | endoplasmic reticulum retention signal, mouse antibody, 25 µgAS10 683 | anti-HDEL | endoplasmic reticulum retention signal, mouse antibody, 100 µgcollection of antibodies for endomembrane proteinsPlant and algal protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1:1000 (WB)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
human, mouse, ratDetects KDEL proteins, GRP94, Grp78, PDI and calreticulin. It may also see ERp57 and ERp29.
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Possible reactivity
other species
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
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References
to be added when available, antibody released in October 2015.
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Scientific context
KDEL (lys-asp-glu-leu) is a C-terminal protein involved in the endoplasmatic reticulum retention system and appears to be sufficient to reduce secretion from the ER.This antibody is directly conjugated to FITC.
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Notes
Immunogen affinity purified antibodies are present in PBS, pH 7.2 with 0.09 % sodium azide and 50 % glycerol.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
This agisera Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. agisera FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
