ASH2 polyclonal antibody

• Catalog number
  6836-50
• Price
  Please ask
• Size
  50 μl

• Antibody Target
  ASH2
• Target Alternative Name
  ASH2L, ASH2L1, ASH2L2, Bre2
• Antibody Type
  Polyclonal
• Species Reactivities
  Mouse.
• Immunogen Sequence
  Polyclonal antibody raised in rabbit against mouse Ash2 (absent, small, or homeotic 2), using 3 different KLH-conjugated synthetic peptides, 2 containing an amino acid sequence from the central and 1 containing an amino acid sequence from the C-terminal part of the protein.
• Accession
  Q9UBL3
• Gene Id
  9070
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS with 0.05% (W/V) sodium azide.
• Purification
  Whole Anti-Sera
• Application
  Western blot, ELISA.
• Positive Control
  Western blot: mouse fibroblasts and neural stem cells, ELISA: Peptides, IF: NIH3T3 cells.
• Application And Usages
  ELISA: 1:100 – 1:500, Western Blotting: 1:500 – 1:1000, IF: 1:200.
• Description
  Ash2 is a component of the Set1/Ash2 histone methyltransferase (HMT) complex. This complex specifically methylates K4 of histone H3, thereby activating transcription. Methylation of K4 is blocked by premethylation of the neighboring K9, a repressor of transcription. This indicates that the Set1/Ash2 HMT complex mediates the crosstalk between K9 methylation and K4 methylation. Ash2 plays a role in hematopoiesis and may be associated with some kinds of leukemia.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Not Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Verified
• ChIP Verified
  Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  ASH2  
• Gene symbol
  ASCL2, ASH2L
• Short name
  ASH2 polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Rabbit IgG
• Alternative name
  ASH2 polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:739
• Gene
  ASCL2
• Long gene name
  achaete-scute family bHLH transcription factor 2
• Synonyms gene name
  • achaete-scute complex (Drosophila) homolog-like 2
  • achaete-scute complex-like 2 (Drosophila)
  • achaete-scute complex homolog 2 (Drosophila)
• Synonyms
  • ASH2
  • HASH2
  • bHLHa45
• Locus
  11p15.5
• Discovery year
  1996-04-12
• Entrez gene record
  430
• Pubmed identfication
  • 9175731
• RefSeq identity
  • NM_005170
• Classification
  • Basic helix-loop-helix proteins
• VEGA ID
  OTTHUMG00000009648

Gene info

• Identity
  HGNC:744
• Gene
  ASH2L
• Long gene name
  ASH2 like, histone lysine methyltransferase complex subunit
• Synonyms gene
  • ASH2L1
• Synonyms gene name
  • ash2 (absent, small, or homeotic)-like (Drosophila)
  • ASH2 like histone lysine methyltransferase complex subunit
• Synonyms
  • ASH2L2
  • ASH2
  • Bre2
• GenBank acession
  • AF056717
• Locus
  8p11.23
• Discovery year
  1998-12-03
• Entrez gene record
  9070
• Pubmed identfication
  • 10393421
  • 21660059
• RefSeq identity
  • NM_004674
• Classification
  • WRAD complex
  • PHD finger proteins
• VEGA ID
  OTTHUMG00000164016

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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