EZViable™ Calcein AM Cell Viability Assay Kit (Fluorometric)

• Catalog number
  K305-1000
• Price
  Please ask
• Size
  1000 assays

• Description
  High-throughput, detects as low as 50 viable cells in less than 30 min.
• Summary
  • Detection method- Fluorescence (Ex/Em = 485/530 nm) • Applications- - Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients - Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals - Assessment of physiological mediators and antibodies that affect cell growth.
• Detection Method
  Fluorescence (Ex/Em = 485/530 nm)
• Applications
  • Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients • Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals • Assessment of physiological mediators and antibodies that affect cell growth.
• Sample Type
  • Adherent and suspension cells • Proliferating and non-proliferating cells
• Features Benefits
  • Convenient & non-radioactive • High-throughput • Highly-sensitive: detect as low as 50 viable cells in less than 30 min. • Suitable for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Quantification of number of viable cells is an indispensible tool in Cell Biology research. BioVision’s Calcein AM Cell Viability Assay Kit is a fluorometric method for extremely sensitive quantification of viable cells that can detect as low as 50 viable cells in less than 30 min. Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells. Hydrolysis of Calcein AM by intracellular esterase produces a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  EZViable™   Calcein   Viability   Kit   Fluorometric  
• Short name
  EZViable™ Calcein AM Viability Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  EZViable™ Calcein AM cellular cytotoxity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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