PI3 kinase (p110a/p85a), Active Human recombinant protein FLAG tag

• Catalog number
  GWB-F64C10
• Price
  Please ask
• Size
  1 vial

• Storage temperature
  store cold
• Expiry date
  1 y
• Category
  research antibodies, ELISAs, recombinant proteins, vectors and reagents for laboratory use
• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Flag
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PI3   kinase   p110a/p85a   ActiveFLAG   tag  
• Gene symbol
  PI3
• Short name
  PI3 kinase (p110a/p85a), Active recombinant protein tag
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. genways advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  Flag
• Species
  Human, Humans
• Alternative name
  PI3 phosphorylation catalyst (p110a/p85a), functionnal H. sapiens Rec. protein FLAG detection labelled
• Alternative technique
  rec

Gene info

• Identity
  HGNC:8947
• Gene
  PI3
• Long gene name
  peptidase inhibitor 3
• Synonyms gene name
  • protease inhibitor 3, skin-derived (SKALP)
  • peptidase inhibitor 3, skin-derived
• Synonyms
  • ESI
  • SKALP
  • ELAFIN
  • WAP3
  • WFDC14
  • cementoin
• Synonyms name
  • skin-derived antileukoproteinase
  • trappin-2
• GenBank acession
  • D13156
• Locus
  20q13.12
• Discovery year
  1993-07-27
• Entrez gene record
  5266
• Pubmed identfication
  • 8287685
  • 12206714
• RefSeq identity
  • NM_002638
• Classification
  • WAP four-disulfide core domain containing
• VEGA ID
  OTTHUMG00000032567

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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